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Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand

Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of Blastocystis in villagers living along the Chao Phraya River, Ayutthaya Province, Thailand, and...

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Autores principales: Palasuwan, Attakorn, Palasuwan, Duangdao, Mahittikorn, Aongart, Chiabchalard, Rachatawan, Combes, Valery, Popruk, Supaluk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040084/
https://www.ncbi.nlm.nih.gov/pubmed/27658597
http://dx.doi.org/10.3347/kjp.2016.54.4.455
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author Palasuwan, Attakorn
Palasuwan, Duangdao
Mahittikorn, Aongart
Chiabchalard, Rachatawan
Combes, Valery
Popruk, Supaluk
author_facet Palasuwan, Attakorn
Palasuwan, Duangdao
Mahittikorn, Aongart
Chiabchalard, Rachatawan
Combes, Valery
Popruk, Supaluk
author_sort Palasuwan, Attakorn
collection PubMed
description Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of Blastocystis in villagers living along the Chao Phraya River, Ayutthaya Province, Thailand, and to assess the risk of zoonotic infection. In total, 220 stool samples were collected, and DNA was extracted. PCR and sequencing were performed with primers targeting the small-subunit ribosomal RNA (SSU rRNA) genes. Blastocystis was present in 5.9% (13/220) of samples, and ST3 (5.0%; 11/220) was the predominant subtype, followed by ST2 (0.45%; 1/220) and ST6 (0.45%; 1/220). Phylogenetic trees were constructed with the maximum-likelihood method based on the Hasegawa–Kishino–Yano + G + I model, neighbor-joining, and maximum parsimony methods. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. All the sequences of the Blastocystis-positive samples (KU051524–KU051536) were closely related to those from animals (pig, cattle, and chicken), indicating a zoonotic risk. Therefore, the villagers require proper health education, especially regarding the prevention of parasitic infection, to improve their personal hygiene and community health. Further studies are required to investigate the Blastocystis STs in the animals living in these villages.
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spelling pubmed-50400842016-09-29 Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand Palasuwan, Attakorn Palasuwan, Duangdao Mahittikorn, Aongart Chiabchalard, Rachatawan Combes, Valery Popruk, Supaluk Korean J Parasitol Original Article Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of Blastocystis in villagers living along the Chao Phraya River, Ayutthaya Province, Thailand, and to assess the risk of zoonotic infection. In total, 220 stool samples were collected, and DNA was extracted. PCR and sequencing were performed with primers targeting the small-subunit ribosomal RNA (SSU rRNA) genes. Blastocystis was present in 5.9% (13/220) of samples, and ST3 (5.0%; 11/220) was the predominant subtype, followed by ST2 (0.45%; 1/220) and ST6 (0.45%; 1/220). Phylogenetic trees were constructed with the maximum-likelihood method based on the Hasegawa–Kishino–Yano + G + I model, neighbor-joining, and maximum parsimony methods. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. All the sequences of the Blastocystis-positive samples (KU051524–KU051536) were closely related to those from animals (pig, cattle, and chicken), indicating a zoonotic risk. Therefore, the villagers require proper health education, especially regarding the prevention of parasitic infection, to improve their personal hygiene and community health. Further studies are required to investigate the Blastocystis STs in the animals living in these villages. The Korean Society for Parasitology and Tropical Medicine 2016-08 2016-08-31 /pmc/articles/PMC5040084/ /pubmed/27658597 http://dx.doi.org/10.3347/kjp.2016.54.4.455 Text en © 2016, Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Palasuwan, Attakorn
Palasuwan, Duangdao
Mahittikorn, Aongart
Chiabchalard, Rachatawan
Combes, Valery
Popruk, Supaluk
Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand
title Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand
title_full Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand
title_fullStr Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand
title_full_unstemmed Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand
title_short Subtype Distribution of Blastocystis in Communities along the Chao Phraya River, Thailand
title_sort subtype distribution of blastocystis in communities along the chao phraya river, thailand
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040084/
https://www.ncbi.nlm.nih.gov/pubmed/27658597
http://dx.doi.org/10.3347/kjp.2016.54.4.455
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