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In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium

There is renewed interest in natural products as a starting point for discovery of drugs for schistosomiasis. Recent studies have shown that phytol reveals interesting in vivo and in vitro antischistosomal properties against Schistosoma mansoni adult worms. Here, we report the in vitro antischistoso...

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Autores principales: Eraky, Maysa Ahmad, Aly, Nagwa Shaban Mohamed, Selem, Rabab Fawzy, El-Kholy, Asmaa Abd El-Monem, Rashed, Gehan Abd El-Rahman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040092/
https://www.ncbi.nlm.nih.gov/pubmed/27658600
http://dx.doi.org/10.3347/kjp.2016.54.4.477
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author Eraky, Maysa Ahmad
Aly, Nagwa Shaban Mohamed
Selem, Rabab Fawzy
El-Kholy, Asmaa Abd El-Monem
Rashed, Gehan Abd El-Rahman
author_facet Eraky, Maysa Ahmad
Aly, Nagwa Shaban Mohamed
Selem, Rabab Fawzy
El-Kholy, Asmaa Abd El-Monem
Rashed, Gehan Abd El-Rahman
author_sort Eraky, Maysa Ahmad
collection PubMed
description There is renewed interest in natural products as a starting point for discovery of drugs for schistosomiasis. Recent studies have shown that phytol reveals interesting in vivo and in vitro antischistosomal properties against Schistosoma mansoni adult worms. Here, we report the in vitro antischistosomal activity of phytol against Schistosoma haematobium juvenile and adult worms and alterations on the tegumental surface of the worms by means of scanning electron microscopy. The assay, which was carried out with 6 concentrations (25, 50, 75, 100, 125, and 150 μg/ml) of phytol, has shown a promising activity in a dose and time-dependent manner. There was a significant decline in the motility of the worms and a mortality rate of 100% was found at 48 hr after they had been exposed to phytol in the concentration of 150 μg/ml. Male worms were more susceptible. On the ultrastructural level, phytol also induced tegumental peeling, disintegration of tubercles and spines in addition to morphological disfiguring of the oral and ventral suckers. This report provides the first evidence that phytol is able to kill S. haematobium of different ages, and emphasizes that it is a promising natural product that could be used for development of a new schistosomicidal agent.
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spelling pubmed-50400922016-09-29 In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium Eraky, Maysa Ahmad Aly, Nagwa Shaban Mohamed Selem, Rabab Fawzy El-Kholy, Asmaa Abd El-Monem Rashed, Gehan Abd El-Rahman Korean J Parasitol Original Article There is renewed interest in natural products as a starting point for discovery of drugs for schistosomiasis. Recent studies have shown that phytol reveals interesting in vivo and in vitro antischistosomal properties against Schistosoma mansoni adult worms. Here, we report the in vitro antischistosomal activity of phytol against Schistosoma haematobium juvenile and adult worms and alterations on the tegumental surface of the worms by means of scanning electron microscopy. The assay, which was carried out with 6 concentrations (25, 50, 75, 100, 125, and 150 μg/ml) of phytol, has shown a promising activity in a dose and time-dependent manner. There was a significant decline in the motility of the worms and a mortality rate of 100% was found at 48 hr after they had been exposed to phytol in the concentration of 150 μg/ml. Male worms were more susceptible. On the ultrastructural level, phytol also induced tegumental peeling, disintegration of tubercles and spines in addition to morphological disfiguring of the oral and ventral suckers. This report provides the first evidence that phytol is able to kill S. haematobium of different ages, and emphasizes that it is a promising natural product that could be used for development of a new schistosomicidal agent. The Korean Society for Parasitology and Tropical Medicine 2016-08 2016-08-31 /pmc/articles/PMC5040092/ /pubmed/27658600 http://dx.doi.org/10.3347/kjp.2016.54.4.477 Text en © 2016, Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Eraky, Maysa Ahmad
Aly, Nagwa Shaban Mohamed
Selem, Rabab Fawzy
El-Kholy, Asmaa Abd El-Monem
Rashed, Gehan Abd El-Rahman
In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium
title In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium
title_full In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium
title_fullStr In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium
title_full_unstemmed In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium
title_short In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium
title_sort in vitro schistosomicidal activity of phytol and tegumental alterations induced in juvenile and adult stages of schistosoma haematobium
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040092/
https://www.ncbi.nlm.nih.gov/pubmed/27658600
http://dx.doi.org/10.3347/kjp.2016.54.4.477
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