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A Brucella spp. Isolate from a Pac-Man Frog (Ceratophrys ornata) Reveals Characteristics Departing from Classical Brucellae

Brucella are highly infectious bacterial pathogens responsible for brucellosis, a frequent worldwide zoonosis. The Brucella genus has recently expanded from 6 to 11 species, all of which were associated with mammals; The natural host range recently expanded to amphibians after some reports of atypic...

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Autores principales: Soler-Lloréns, Pedro F., Quance, Chris R., Lawhon, Sara D., Stuber, Tod P., Edwards, John F., Ficht, Thomas A., Robbe-Austerman, Suelee, O'Callaghan, David, Keriel, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040101/
https://www.ncbi.nlm.nih.gov/pubmed/27734009
http://dx.doi.org/10.3389/fcimb.2016.00116
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author Soler-Lloréns, Pedro F.
Quance, Chris R.
Lawhon, Sara D.
Stuber, Tod P.
Edwards, John F.
Ficht, Thomas A.
Robbe-Austerman, Suelee
O'Callaghan, David
Keriel, Anne
author_facet Soler-Lloréns, Pedro F.
Quance, Chris R.
Lawhon, Sara D.
Stuber, Tod P.
Edwards, John F.
Ficht, Thomas A.
Robbe-Austerman, Suelee
O'Callaghan, David
Keriel, Anne
author_sort Soler-Lloréns, Pedro F.
collection PubMed
description Brucella are highly infectious bacterial pathogens responsible for brucellosis, a frequent worldwide zoonosis. The Brucella genus has recently expanded from 6 to 11 species, all of which were associated with mammals; The natural host range recently expanded to amphibians after some reports of atypical strains from frogs. Here we describe the first in depth phenotypic and genetic characterization of a Brucella strains isolated from a frog. Strain B13-0095 was isolated from a Pac-Man frog (Ceratophyrus ornate) at a veterinary hospital in Texas and was initially misidentified as Ochrobactrum anthropi. We found that B13-0095 belongs to a group of early-diverging brucellae that includes Brucella inopinata strain BO1 and the B. inopinata-like strain BO2, with traits that depart significantly from those of the “classical” Brucella spp. Analysis of B13-0095 genome sequence revealed several specific features that suggest that this isolate represents an intermediate between a soil associated ancestor and the host adapted “classical” species. Like strain BO2, B13-0095 does not possess the genes required to produce the perosamine based LPS found in classical Brucella, but has a set of genes that could encode a rhamnose based O-antigen. Despite this, B13-0095 has a very fast intracellular replication rate in both epithelial cells and macrophages. Finally, another major finding in this study is the bacterial motility observed for strains B13-0095, BO1, and BO2, which is remarkable for this bacterial genus. This study thus highlights several novel characteristics in strains belonging to an emerging group within the Brucella genus. Accurate identification tools for such atypical Brucella isolates and careful evaluation of their zoonotic potential, are urgently required.
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spelling pubmed-50401012016-10-12 A Brucella spp. Isolate from a Pac-Man Frog (Ceratophrys ornata) Reveals Characteristics Departing from Classical Brucellae Soler-Lloréns, Pedro F. Quance, Chris R. Lawhon, Sara D. Stuber, Tod P. Edwards, John F. Ficht, Thomas A. Robbe-Austerman, Suelee O'Callaghan, David Keriel, Anne Front Cell Infect Microbiol Microbiology Brucella are highly infectious bacterial pathogens responsible for brucellosis, a frequent worldwide zoonosis. The Brucella genus has recently expanded from 6 to 11 species, all of which were associated with mammals; The natural host range recently expanded to amphibians after some reports of atypical strains from frogs. Here we describe the first in depth phenotypic and genetic characterization of a Brucella strains isolated from a frog. Strain B13-0095 was isolated from a Pac-Man frog (Ceratophyrus ornate) at a veterinary hospital in Texas and was initially misidentified as Ochrobactrum anthropi. We found that B13-0095 belongs to a group of early-diverging brucellae that includes Brucella inopinata strain BO1 and the B. inopinata-like strain BO2, with traits that depart significantly from those of the “classical” Brucella spp. Analysis of B13-0095 genome sequence revealed several specific features that suggest that this isolate represents an intermediate between a soil associated ancestor and the host adapted “classical” species. Like strain BO2, B13-0095 does not possess the genes required to produce the perosamine based LPS found in classical Brucella, but has a set of genes that could encode a rhamnose based O-antigen. Despite this, B13-0095 has a very fast intracellular replication rate in both epithelial cells and macrophages. Finally, another major finding in this study is the bacterial motility observed for strains B13-0095, BO1, and BO2, which is remarkable for this bacterial genus. This study thus highlights several novel characteristics in strains belonging to an emerging group within the Brucella genus. Accurate identification tools for such atypical Brucella isolates and careful evaluation of their zoonotic potential, are urgently required. Frontiers Media S.A. 2016-09-28 /pmc/articles/PMC5040101/ /pubmed/27734009 http://dx.doi.org/10.3389/fcimb.2016.00116 Text en Copyright © 2016 Soler-Lloréns, Quance, Lawhon, Stuber, Edwards, Ficht, Robbe-Austerman, O'Callaghan and Keriel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Soler-Lloréns, Pedro F.
Quance, Chris R.
Lawhon, Sara D.
Stuber, Tod P.
Edwards, John F.
Ficht, Thomas A.
Robbe-Austerman, Suelee
O'Callaghan, David
Keriel, Anne
A Brucella spp. Isolate from a Pac-Man Frog (Ceratophrys ornata) Reveals Characteristics Departing from Classical Brucellae
title A Brucella spp. Isolate from a Pac-Man Frog (Ceratophrys ornata) Reveals Characteristics Departing from Classical Brucellae
title_full A Brucella spp. Isolate from a Pac-Man Frog (Ceratophrys ornata) Reveals Characteristics Departing from Classical Brucellae
title_fullStr A Brucella spp. Isolate from a Pac-Man Frog (Ceratophrys ornata) Reveals Characteristics Departing from Classical Brucellae
title_full_unstemmed A Brucella spp. Isolate from a Pac-Man Frog (Ceratophrys ornata) Reveals Characteristics Departing from Classical Brucellae
title_short A Brucella spp. Isolate from a Pac-Man Frog (Ceratophrys ornata) Reveals Characteristics Departing from Classical Brucellae
title_sort brucella spp. isolate from a pac-man frog (ceratophrys ornata) reveals characteristics departing from classical brucellae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040101/
https://www.ncbi.nlm.nih.gov/pubmed/27734009
http://dx.doi.org/10.3389/fcimb.2016.00116
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