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Re-engineering a NiFe hydrogenase to increase the H(2) production bias while maintaining native levels of O(2) tolerance
Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H(2) production to double but...
Autores principales: | Flanagan, Lindsey A., Wright, John J., Roessler, Maxie M., Moir, James W., Parkin, Alison |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040170/ https://www.ncbi.nlm.nih.gov/pubmed/27055899 http://dx.doi.org/10.1039/c6cc00515b |
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