Cargando…
A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis
Experiments of cell migration and chemotaxis assays have been classically performed in the so-called Boyden Chambers. A recent technology, xCELLigence Real Time Cell Analysis, is now allowing to monitor the cell migration in real time. This technology measures impedance changes caused by the gradual...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040252/ https://www.ncbi.nlm.nih.gov/pubmed/27680883 http://dx.doi.org/10.1371/journal.pone.0162553 |
_version_ | 1782456210567462912 |
---|---|
author | Di Costanzo, Ezio Ingangi, Vincenzo Angelini, Claudia Carfora, Maria Francesca Carriero, Maria Vincenza Natalini, Roberto |
author_facet | Di Costanzo, Ezio Ingangi, Vincenzo Angelini, Claudia Carfora, Maria Francesca Carriero, Maria Vincenza Natalini, Roberto |
author_sort | Di Costanzo, Ezio |
collection | PubMed |
description | Experiments of cell migration and chemotaxis assays have been classically performed in the so-called Boyden Chambers. A recent technology, xCELLigence Real Time Cell Analysis, is now allowing to monitor the cell migration in real time. This technology measures impedance changes caused by the gradual increase of electrode surface occupation by cells during the course of time and provide a Cell Index which is proportional to cellular morphology, spreading, ruffling and adhesion quality as well as cell number. In this paper we propose a macroscopic mathematical model, based on advection-reaction-diffusion partial differential equations, describing the cell migration assay using the real-time technology. We carried out numerical simulations to compare simulated model dynamics with data of observed biological experiments on three different cell lines and in two experimental settings: absence of chemotactic signals (basal migration) and presence of a chemoattractant. Overall we conclude that our minimal mathematical model is able to describe the phenomenon in the real time scale and numerical results show a good agreement with the experimental evidences. |
format | Online Article Text |
id | pubmed-5040252 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50402522016-10-27 A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis Di Costanzo, Ezio Ingangi, Vincenzo Angelini, Claudia Carfora, Maria Francesca Carriero, Maria Vincenza Natalini, Roberto PLoS One Research Article Experiments of cell migration and chemotaxis assays have been classically performed in the so-called Boyden Chambers. A recent technology, xCELLigence Real Time Cell Analysis, is now allowing to monitor the cell migration in real time. This technology measures impedance changes caused by the gradual increase of electrode surface occupation by cells during the course of time and provide a Cell Index which is proportional to cellular morphology, spreading, ruffling and adhesion quality as well as cell number. In this paper we propose a macroscopic mathematical model, based on advection-reaction-diffusion partial differential equations, describing the cell migration assay using the real-time technology. We carried out numerical simulations to compare simulated model dynamics with data of observed biological experiments on three different cell lines and in two experimental settings: absence of chemotactic signals (basal migration) and presence of a chemoattractant. Overall we conclude that our minimal mathematical model is able to describe the phenomenon in the real time scale and numerical results show a good agreement with the experimental evidences. Public Library of Science 2016-09-28 /pmc/articles/PMC5040252/ /pubmed/27680883 http://dx.doi.org/10.1371/journal.pone.0162553 Text en © 2016 Di Costanzo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Di Costanzo, Ezio Ingangi, Vincenzo Angelini, Claudia Carfora, Maria Francesca Carriero, Maria Vincenza Natalini, Roberto A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis |
title | A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis |
title_full | A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis |
title_fullStr | A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis |
title_full_unstemmed | A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis |
title_short | A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis |
title_sort | macroscopic mathematical model for cell migration assays using a real-time cell analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040252/ https://www.ncbi.nlm.nih.gov/pubmed/27680883 http://dx.doi.org/10.1371/journal.pone.0162553 |
work_keys_str_mv | AT dicostanzoezio amacroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT ingangivincenzo amacroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT angeliniclaudia amacroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT carforamariafrancesca amacroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT carrieromariavincenza amacroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT nataliniroberto amacroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT dicostanzoezio macroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT ingangivincenzo macroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT angeliniclaudia macroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT carforamariafrancesca macroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT carrieromariavincenza macroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis AT nataliniroberto macroscopicmathematicalmodelforcellmigrationassaysusingarealtimecellanalysis |