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Discovery of Novel Viruses in Mosquitoes from the Zambezi Valley of Mozambique

Mosquitoes carry a wide variety of viruses that can cause vector-borne infectious diseases and affect both human and veterinary public health. Although Mozambique can be considered a hot spot for emerging infectious diseases due to factors such as a rich vector population and a close vector/human/wi...

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Autores principales: Cholleti, Harindranath, Hayer, Juliette, Abilio, Ana Paula, Mulandane, Fernando Chanisso, Verner-Carlsson, Jenny, Falk, Kerstin I., Fafetine, Jose M., Berg, Mikael, Blomström, Anne-Lie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040392/
https://www.ncbi.nlm.nih.gov/pubmed/27682810
http://dx.doi.org/10.1371/journal.pone.0162751
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author Cholleti, Harindranath
Hayer, Juliette
Abilio, Ana Paula
Mulandane, Fernando Chanisso
Verner-Carlsson, Jenny
Falk, Kerstin I.
Fafetine, Jose M.
Berg, Mikael
Blomström, Anne-Lie
author_facet Cholleti, Harindranath
Hayer, Juliette
Abilio, Ana Paula
Mulandane, Fernando Chanisso
Verner-Carlsson, Jenny
Falk, Kerstin I.
Fafetine, Jose M.
Berg, Mikael
Blomström, Anne-Lie
author_sort Cholleti, Harindranath
collection PubMed
description Mosquitoes carry a wide variety of viruses that can cause vector-borne infectious diseases and affect both human and veterinary public health. Although Mozambique can be considered a hot spot for emerging infectious diseases due to factors such as a rich vector population and a close vector/human/wildlife interface, the viral flora in mosquitoes have not previously been investigated. In this study, viral metagenomics was employed to analyze the viral communities in Culex and Mansonia mosquitoes in the Zambezia province of Mozambique. Among the 1.7 and 2.6 million sequences produced from the Culex and Mansonia samples, respectively, 3269 and 983 reads were classified as viral sequences. Viruses belonging to the Flaviviridae, Rhabdoviridae and Iflaviridae families were detected, and different unclassified single- and double-stranded RNA viruses were also identified. A near complete genome of a flavivirus, tentatively named Cuacua virus, was obtained from the Mansonia mosquitoes. Phylogenetic analysis of this flavivirus, using the NS5 amino acid sequence, showed that it grouped with ‘insect-specific’ viruses and was most closely related to Nakiwogo virus previously identified in Uganda. Both mosquito genera had viral sequences related to Rhabdoviruses, and these were most closely related to Culex tritaeniorhynchus rhabdovirus (CTRV). The results from this study suggest that several viruses specific for insects belonging to, for example, the Flaviviridae and Rhabdoviridae families, as well as a number of unclassified RNA viruses, are present in mosquitoes in Mozambique.
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spelling pubmed-50403922016-10-27 Discovery of Novel Viruses in Mosquitoes from the Zambezi Valley of Mozambique Cholleti, Harindranath Hayer, Juliette Abilio, Ana Paula Mulandane, Fernando Chanisso Verner-Carlsson, Jenny Falk, Kerstin I. Fafetine, Jose M. Berg, Mikael Blomström, Anne-Lie PLoS One Research Article Mosquitoes carry a wide variety of viruses that can cause vector-borne infectious diseases and affect both human and veterinary public health. Although Mozambique can be considered a hot spot for emerging infectious diseases due to factors such as a rich vector population and a close vector/human/wildlife interface, the viral flora in mosquitoes have not previously been investigated. In this study, viral metagenomics was employed to analyze the viral communities in Culex and Mansonia mosquitoes in the Zambezia province of Mozambique. Among the 1.7 and 2.6 million sequences produced from the Culex and Mansonia samples, respectively, 3269 and 983 reads were classified as viral sequences. Viruses belonging to the Flaviviridae, Rhabdoviridae and Iflaviridae families were detected, and different unclassified single- and double-stranded RNA viruses were also identified. A near complete genome of a flavivirus, tentatively named Cuacua virus, was obtained from the Mansonia mosquitoes. Phylogenetic analysis of this flavivirus, using the NS5 amino acid sequence, showed that it grouped with ‘insect-specific’ viruses and was most closely related to Nakiwogo virus previously identified in Uganda. Both mosquito genera had viral sequences related to Rhabdoviruses, and these were most closely related to Culex tritaeniorhynchus rhabdovirus (CTRV). The results from this study suggest that several viruses specific for insects belonging to, for example, the Flaviviridae and Rhabdoviridae families, as well as a number of unclassified RNA viruses, are present in mosquitoes in Mozambique. Public Library of Science 2016-09-28 /pmc/articles/PMC5040392/ /pubmed/27682810 http://dx.doi.org/10.1371/journal.pone.0162751 Text en © 2016 Cholleti et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cholleti, Harindranath
Hayer, Juliette
Abilio, Ana Paula
Mulandane, Fernando Chanisso
Verner-Carlsson, Jenny
Falk, Kerstin I.
Fafetine, Jose M.
Berg, Mikael
Blomström, Anne-Lie
Discovery of Novel Viruses in Mosquitoes from the Zambezi Valley of Mozambique
title Discovery of Novel Viruses in Mosquitoes from the Zambezi Valley of Mozambique
title_full Discovery of Novel Viruses in Mosquitoes from the Zambezi Valley of Mozambique
title_fullStr Discovery of Novel Viruses in Mosquitoes from the Zambezi Valley of Mozambique
title_full_unstemmed Discovery of Novel Viruses in Mosquitoes from the Zambezi Valley of Mozambique
title_short Discovery of Novel Viruses in Mosquitoes from the Zambezi Valley of Mozambique
title_sort discovery of novel viruses in mosquitoes from the zambezi valley of mozambique
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040392/
https://www.ncbi.nlm.nih.gov/pubmed/27682810
http://dx.doi.org/10.1371/journal.pone.0162751
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