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MgaSpn and H-NS: Two Unrelated Global Regulators with Similar DNA-Binding Properties

Global regulators play an essential role in the adaptation of bacterial cells to specific niches. Bacterial pathogens thriving in the tissues and organs of their eukaryotic hosts are a well-studied example. Some of the proteins that recognize local DNA structures rather than specific nucleotide sequ...

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Autores principales: Solano-Collado, Virtu, Hüttener, Mário, Espinosa, Manuel, Juárez, Antonio, Bravo, Alicia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040716/
https://www.ncbi.nlm.nih.gov/pubmed/27747214
http://dx.doi.org/10.3389/fmolb.2016.00060
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author Solano-Collado, Virtu
Hüttener, Mário
Espinosa, Manuel
Juárez, Antonio
Bravo, Alicia
author_facet Solano-Collado, Virtu
Hüttener, Mário
Espinosa, Manuel
Juárez, Antonio
Bravo, Alicia
author_sort Solano-Collado, Virtu
collection PubMed
description Global regulators play an essential role in the adaptation of bacterial cells to specific niches. Bacterial pathogens thriving in the tissues and organs of their eukaryotic hosts are a well-studied example. Some of the proteins that recognize local DNA structures rather than specific nucleotide sequences act as global modulators in many bacteria, both Gram-negative and -positive. To this class of regulators belong the H-NS-like proteins, mainly identified in γ-Proteobacteria, and the MgaSpn-like proteins identified in Firmicutes. H-NS and MgaSpn from Escherichia coli and Streptococcus pneumoniae, respectively, neither have sequence similarity nor share structural domains. Nevertheless, they display common features in their interaction with DNA, namely: (i) they bind to DNA in a non-sequence-specific manner, (ii) they have a preference for intrinsically curved DNA regions, and (iii) they are able to form multimeric complexes on linear DNA. Using DNA fragments from the hemolysin operon regulatory region of the E. coli plasmid pHly152, we show in this work that MgaSpn is able to recognize particular regions on extended H-NS binding sites. Such regions are either located at or flanked by regions of potential bendability. Moreover, we show that the regulatory region of the pneumococcal P1623B promoter, which is recognized by MgaSpn, contains DNA motifs that are recognized by H-NS. These motifs are adjacent to regions of potential bendability. Our results suggest that both regulatory proteins recognize similar structural characteristics of DNA.
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spelling pubmed-50407162016-10-14 MgaSpn and H-NS: Two Unrelated Global Regulators with Similar DNA-Binding Properties Solano-Collado, Virtu Hüttener, Mário Espinosa, Manuel Juárez, Antonio Bravo, Alicia Front Mol Biosci Molecular Biosciences Global regulators play an essential role in the adaptation of bacterial cells to specific niches. Bacterial pathogens thriving in the tissues and organs of their eukaryotic hosts are a well-studied example. Some of the proteins that recognize local DNA structures rather than specific nucleotide sequences act as global modulators in many bacteria, both Gram-negative and -positive. To this class of regulators belong the H-NS-like proteins, mainly identified in γ-Proteobacteria, and the MgaSpn-like proteins identified in Firmicutes. H-NS and MgaSpn from Escherichia coli and Streptococcus pneumoniae, respectively, neither have sequence similarity nor share structural domains. Nevertheless, they display common features in their interaction with DNA, namely: (i) they bind to DNA in a non-sequence-specific manner, (ii) they have a preference for intrinsically curved DNA regions, and (iii) they are able to form multimeric complexes on linear DNA. Using DNA fragments from the hemolysin operon regulatory region of the E. coli plasmid pHly152, we show in this work that MgaSpn is able to recognize particular regions on extended H-NS binding sites. Such regions are either located at or flanked by regions of potential bendability. Moreover, we show that the regulatory region of the pneumococcal P1623B promoter, which is recognized by MgaSpn, contains DNA motifs that are recognized by H-NS. These motifs are adjacent to regions of potential bendability. Our results suggest that both regulatory proteins recognize similar structural characteristics of DNA. Frontiers Media S.A. 2016-09-29 /pmc/articles/PMC5040716/ /pubmed/27747214 http://dx.doi.org/10.3389/fmolb.2016.00060 Text en Copyright © 2016 Solano-Collado, Hüttener, Espinosa, Juárez and Bravo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Solano-Collado, Virtu
Hüttener, Mário
Espinosa, Manuel
Juárez, Antonio
Bravo, Alicia
MgaSpn and H-NS: Two Unrelated Global Regulators with Similar DNA-Binding Properties
title MgaSpn and H-NS: Two Unrelated Global Regulators with Similar DNA-Binding Properties
title_full MgaSpn and H-NS: Two Unrelated Global Regulators with Similar DNA-Binding Properties
title_fullStr MgaSpn and H-NS: Two Unrelated Global Regulators with Similar DNA-Binding Properties
title_full_unstemmed MgaSpn and H-NS: Two Unrelated Global Regulators with Similar DNA-Binding Properties
title_short MgaSpn and H-NS: Two Unrelated Global Regulators with Similar DNA-Binding Properties
title_sort mgaspn and h-ns: two unrelated global regulators with similar dna-binding properties
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040716/
https://www.ncbi.nlm.nih.gov/pubmed/27747214
http://dx.doi.org/10.3389/fmolb.2016.00060
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