Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning

Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres t...

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Autores principales: Saallah, Suryani, Naim, M. Nazli, Lenggoro, I. Wuled, Mokhtar, Mohd Noriznan, Abu Bakar, Noor Fitrah, Gen, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040860/
https://www.ncbi.nlm.nih.gov/pubmed/28352523
http://dx.doi.org/10.1016/j.btre.2016.03.003
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author Saallah, Suryani
Naim, M. Nazli
Lenggoro, I. Wuled
Mokhtar, Mohd Noriznan
Abu Bakar, Noor Fitrah
Gen, Masao
author_facet Saallah, Suryani
Naim, M. Nazli
Lenggoro, I. Wuled
Mokhtar, Mohd Noriznan
Abu Bakar, Noor Fitrah
Gen, Masao
author_sort Saallah, Suryani
collection PubMed
description Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film.
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spelling pubmed-50408602017-03-28 Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning Saallah, Suryani Naim, M. Nazli Lenggoro, I. Wuled Mokhtar, Mohd Noriznan Abu Bakar, Noor Fitrah Gen, Masao Biotechnol Rep (Amst) Article Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film. Elsevier 2016-03-09 /pmc/articles/PMC5040860/ /pubmed/28352523 http://dx.doi.org/10.1016/j.btre.2016.03.003 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Saallah, Suryani
Naim, M. Nazli
Lenggoro, I. Wuled
Mokhtar, Mohd Noriznan
Abu Bakar, Noor Fitrah
Gen, Masao
Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
title Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
title_full Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
title_fullStr Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
title_full_unstemmed Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
title_short Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
title_sort immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (pva) nanofibres via electrospinning
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040860/
https://www.ncbi.nlm.nih.gov/pubmed/28352523
http://dx.doi.org/10.1016/j.btre.2016.03.003
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