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Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species
Callus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regenerati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040870/ https://www.ncbi.nlm.nih.gov/pubmed/28352526 http://dx.doi.org/10.1016/j.btre.2016.03.006 |
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author | Verma, Sandeep Kumar Das, Ashok Kumar Cingoz, Gunce Sahin Uslu, Emel Gurel, Ekrem |
author_facet | Verma, Sandeep Kumar Das, Ashok Kumar Cingoz, Gunce Sahin Uslu, Emel Gurel, Ekrem |
author_sort | Verma, Sandeep Kumar |
collection | PubMed |
description | Callus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regeneration (70%, with 7.2 shoots/callus) were found in the crocus species Crocus oliveri ssp. Oliveri, using the MS medium containing 5% (w/v) sucrose supplemented with (4 mg/L NAA + 4 mg/L TDZ) and (2 mg/L IAA + 2 mg/L TDZ + 2 mg/L BAP). When the embryogenic calli were transferred into the four nutrient media containing (2 mg/L IAA + 2 mg/L TDZ) and 100 mg/L ABA, these further developed into cotyledonary embryos. Maximum number of somatic embryos (2.9 embryos per leaf explant, with a frequency 46.6%) was obtained in C. oliveri ssp. Oliveri. During subculture using the half strength media, cotyledonary embryos gradually developed into plantlets. |
format | Online Article Text |
id | pubmed-5040870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-50408702017-03-28 Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species Verma, Sandeep Kumar Das, Ashok Kumar Cingoz, Gunce Sahin Uslu, Emel Gurel, Ekrem Biotechnol Rep (Amst) Article Callus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regeneration (70%, with 7.2 shoots/callus) were found in the crocus species Crocus oliveri ssp. Oliveri, using the MS medium containing 5% (w/v) sucrose supplemented with (4 mg/L NAA + 4 mg/L TDZ) and (2 mg/L IAA + 2 mg/L TDZ + 2 mg/L BAP). When the embryogenic calli were transferred into the four nutrient media containing (2 mg/L IAA + 2 mg/L TDZ) and 100 mg/L ABA, these further developed into cotyledonary embryos. Maximum number of somatic embryos (2.9 embryos per leaf explant, with a frequency 46.6%) was obtained in C. oliveri ssp. Oliveri. During subculture using the half strength media, cotyledonary embryos gradually developed into plantlets. Elsevier 2016-03-26 /pmc/articles/PMC5040870/ /pubmed/28352526 http://dx.doi.org/10.1016/j.btre.2016.03.006 Text en © 2016 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Verma, Sandeep Kumar Das, Ashok Kumar Cingoz, Gunce Sahin Uslu, Emel Gurel, Ekrem Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species |
title | Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species |
title_full | Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species |
title_fullStr | Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species |
title_full_unstemmed | Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species |
title_short | Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species |
title_sort | influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected turkish crocus species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040870/ https://www.ncbi.nlm.nih.gov/pubmed/28352526 http://dx.doi.org/10.1016/j.btre.2016.03.006 |
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