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Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles

ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe(3)O(4) nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magne...

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Detalles Bibliográficos
Autores principales: Jia, Honghua, Huang, Fan, Gao, Zhen, Zhong, Chao, Zhou, Hua, Jiang, Min, Wei, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040871/
https://www.ncbi.nlm.nih.gov/pubmed/28352524
http://dx.doi.org/10.1016/j.btre.2016.03.004
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author Jia, Honghua
Huang, Fan
Gao, Zhen
Zhong, Chao
Zhou, Hua
Jiang, Min
Wei, Ping
author_facet Jia, Honghua
Huang, Fan
Gao, Zhen
Zhong, Chao
Zhou, Hua
Jiang, Min
Wei, Ping
author_sort Jia, Honghua
collection PubMed
description ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe(3)O(4) nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic PVA-Fe(3)O(4) nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The average size of magnetic PVA-Fe(3)O(4) nanoparticles was located between 30–40 nm ω-TA was immobilized onto magnetic PVA-Fe(3)O(4) nanoparticles via glutaraldehyde cross-linking, achieving a wider pH range as 6–8 and also a better thermal stability at 60 °C. Meanwhile, the immobilized ω-TA could be successfully reused for 13 times in biotransformation. These results therefore indicated that the immobilized ω-TA with high stability might be readily utilized in industrial purposes.
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spelling pubmed-50408712017-03-28 Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles Jia, Honghua Huang, Fan Gao, Zhen Zhong, Chao Zhou, Hua Jiang, Min Wei, Ping Biotechnol Rep (Amst) Article ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe(3)O(4) nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic PVA-Fe(3)O(4) nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The average size of magnetic PVA-Fe(3)O(4) nanoparticles was located between 30–40 nm ω-TA was immobilized onto magnetic PVA-Fe(3)O(4) nanoparticles via glutaraldehyde cross-linking, achieving a wider pH range as 6–8 and also a better thermal stability at 60 °C. Meanwhile, the immobilized ω-TA could be successfully reused for 13 times in biotransformation. These results therefore indicated that the immobilized ω-TA with high stability might be readily utilized in industrial purposes. Elsevier 2016-03-11 /pmc/articles/PMC5040871/ /pubmed/28352524 http://dx.doi.org/10.1016/j.btre.2016.03.004 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Jia, Honghua
Huang, Fan
Gao, Zhen
Zhong, Chao
Zhou, Hua
Jiang, Min
Wei, Ping
Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles
title Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles
title_full Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles
title_fullStr Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles
title_full_unstemmed Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles
title_short Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles
title_sort immobilization of ω-transaminase by magnetic pva-fe(3)o(4) nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040871/
https://www.ncbi.nlm.nih.gov/pubmed/28352524
http://dx.doi.org/10.1016/j.btre.2016.03.004
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