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Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles
ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe(3)O(4) nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magne...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040871/ https://www.ncbi.nlm.nih.gov/pubmed/28352524 http://dx.doi.org/10.1016/j.btre.2016.03.004 |
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author | Jia, Honghua Huang, Fan Gao, Zhen Zhong, Chao Zhou, Hua Jiang, Min Wei, Ping |
author_facet | Jia, Honghua Huang, Fan Gao, Zhen Zhong, Chao Zhou, Hua Jiang, Min Wei, Ping |
author_sort | Jia, Honghua |
collection | PubMed |
description | ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe(3)O(4) nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic PVA-Fe(3)O(4) nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The average size of magnetic PVA-Fe(3)O(4) nanoparticles was located between 30–40 nm ω-TA was immobilized onto magnetic PVA-Fe(3)O(4) nanoparticles via glutaraldehyde cross-linking, achieving a wider pH range as 6–8 and also a better thermal stability at 60 °C. Meanwhile, the immobilized ω-TA could be successfully reused for 13 times in biotransformation. These results therefore indicated that the immobilized ω-TA with high stability might be readily utilized in industrial purposes. |
format | Online Article Text |
id | pubmed-5040871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-50408712017-03-28 Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles Jia, Honghua Huang, Fan Gao, Zhen Zhong, Chao Zhou, Hua Jiang, Min Wei, Ping Biotechnol Rep (Amst) Article ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe(3)O(4) nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic PVA-Fe(3)O(4) nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The average size of magnetic PVA-Fe(3)O(4) nanoparticles was located between 30–40 nm ω-TA was immobilized onto magnetic PVA-Fe(3)O(4) nanoparticles via glutaraldehyde cross-linking, achieving a wider pH range as 6–8 and also a better thermal stability at 60 °C. Meanwhile, the immobilized ω-TA could be successfully reused for 13 times in biotransformation. These results therefore indicated that the immobilized ω-TA with high stability might be readily utilized in industrial purposes. Elsevier 2016-03-11 /pmc/articles/PMC5040871/ /pubmed/28352524 http://dx.doi.org/10.1016/j.btre.2016.03.004 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Jia, Honghua Huang, Fan Gao, Zhen Zhong, Chao Zhou, Hua Jiang, Min Wei, Ping Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles |
title | Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles |
title_full | Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles |
title_fullStr | Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles |
title_full_unstemmed | Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles |
title_short | Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles |
title_sort | immobilization of ω-transaminase by magnetic pva-fe(3)o(4) nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5040871/ https://www.ncbi.nlm.nih.gov/pubmed/28352524 http://dx.doi.org/10.1016/j.btre.2016.03.004 |
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