Cargando…

qKAT: a high-throughput qPCR method for KIR gene copy number and haplotype determination

Killer cell immunoglobulin-like receptors (KIRs), expressed on natural killer cells and T cells, have considerable biomedical relevance playing significant roles in immunity, pregnancy and transplantation. The KIR locus is one of the most complex and polymorphic regions of the human genome. Extensiv...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jiang, W., Johnson, C., Simecek, N., López-Álvarez, M. R., Di, D., Trowsdale, J., Traherne, J. A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041586/ https://www.ncbi.nlm.nih.gov/pubmed/27686127 http://dx.doi.org/10.1186/s13073-016-0358-0

Ejemplares similares

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
por: Jayaraman, Jyothi, et al.
Publicado: (2019)

A hybrid qPCR/SNP array approach allows cost efficient assessment of KIR gene copy numbers in large samples
por: Pontikos, Nikolas, et al.
Publicado: (2014)

Copy number variation leads to considerable diversity for B but not A haplotypes of the human KIR genes encoding NK cell receptors
por: Jiang, Wei, et al.
Publicado: (2012)

Killer-cell Immunoglobulin-like Receptor gene linkage and copy number variation analysis by droplet digital PCR
por: Roberts, Chrissy h, et al.
Publicado: (2014)

Laboratory Exercise to Measure Plasmid Copy Number by qPCR
por: David, Benjamin, et al.
Publicado: (2021)

A qPCR Method to Distinguish between Expression of Transgenic and Endogenous Copies of Genes
por: Bezodis, William, et al.
Publicado: (2023)

Accuracy in Copy Number Calling by qPCR and PRT: A Matter of DNA
por: Fernandez-Jimenez, Nora, et al.
Publicado: (2011)

Quantification of siRNA using competitive qPCR
por: Liu, Wei-li, et al.
Publicado: (2009)

Mechanisms of copy number variation and hybrid gene formation in the KIR immune gene complex
por: Traherne, James A., et al.
Publicado: (2010)

A novel and universal method for microRNA RT-qPCR data normalization
por: Mestdagh, Pieter, et al.
Publicado: (2009)

Telomere length measurement by qPCR in birds is affected by storage method of blood samples
por: Reichert, Sophie, et al.
Publicado: (2017)

A novel method for the normalization of ChIP-qPCR data
por: Solomon, Elizabeth R, et al.
Publicado: (2021)

Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR
por: Tan, Ke-En, et al.
Publicado: (2023)

Roadblock-qPCR: a simple and inexpensive strategy for targeted measurements of mRNA stability
por: Watson, Maegan J., et al.
Publicado: (2021)

The ‘PREXCEL-Q Method’ for qPCR
por: Gallup, Jack M., et al.
Publicado: (2008)

High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
por: Mestdagh, Pieter, et al.
Publicado: (2008)

Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
por: Kudo, Eriko, et al.
Publicado: (2020)

qPCR-based characterization of DNA fragmentation efficiency of Tn5 transposomes
por: Rykalina, Vera, et al.
Publicado: (2017)

An improved RT-qPCR method for direct quantification of enveloped RNA viruses
por: Gregorova, Pavlina, et al.
Publicado: (2022)

Practical data handling pipeline improves performance of qPCR-based circulating miRNA measurements
por: de Ronde, Maurice W.J., et al.
Publicado: (2017)

Quantification of microRNA editing using two-tailed RT-qPCR for improved biomarker discovery
por: Voss, Gjendine, et al.
Publicado: (2021)

Genome Size, rDNA Copy, and qPCR Assays for Symbiodiniaceae
por: Saad, Osama S., et al.
Publicado: (2020)

Correction of RT–qPCR data for genomic DNA-derived signals with ValidPrime
por: Laurell, Henrik, et al.
Publicado: (2012)

Evidence‐based selection of reference genes for RT‐qPCR assays in periodontal research
por: Diehl, Daniel, et al.
Publicado: (2022)

Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
por: Shaik, Gouse M., et al.
Publicado: (2008)

Evaluation of critical design parameters for RT‐qPCR‐based analysis of multiple dUTPase isoform genes in mice
por: Rácz, Gergely A., et al.
Publicado: (2019)

Detection of Klebsiella pneumoniae human gut carriage: a comparison of culture, qPCR, and whole metagenomic sequencing methods
por: Lindstedt, Kenneth, et al.
Publicado: (2022)

Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR
por: Maier, Christopher, et al.
Publicado: (2021)

ΩqPCR measures telomere length from single-cells in base pair units
por: Xiong, Fusheng, et al.
Publicado: (2021)

Estimating KIR Haplotype Frequencies on a Cohort of 10,000 Individuals: A Comprehensive Study on Population Variations, Typing Resolutions, and Reference Haplotypes
por: Vierra-Green, Cynthia, et al.
Publicado: (2016)

Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping
por: Li, Baowei, et al.
Publicado: (2021)

Long-range, high-throughput haplotype determination via haplotype-fusion PCR and ligation haplotyping
por: Turner, Daniel J., et al.
Publicado: (2008)

Impartial comparative analysis of measurement of leukocyte telomere length/DNA content by Southern blots and qPCR
por: Aviv, Abraham, et al.
Publicado: (2011)

SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels
por: Barbau-Piednoir, Elodie, et al.
Publicado: (2013)

Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification
por: Androvic, Peter, et al.
Publicado: (2017)

A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection
por: Reijns, Martin A. M., et al.
Publicado: (2020)

Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2
por: Vogels, Chantal B. F., et al.
Publicado: (2021)

OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression
por: He, Fang, et al.
Publicado: (2021)

Large-scale copy number variants (CNVs): Distribution in normal subjects and FISH/real-time qPCR analysis
por: Qiao, Ying, et al.
Publicado: (2007)

Pre-amplification in the context of high-throughput qPCR gene expression experiment
por: Korenková, Vlasta, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_jd64nv9pdhhe06rsb42amr8li3