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XB130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury
XB130 is a novel oncoprotein that promotes cancer cell survival, proliferation and migration. Its physiological function in vivo is largely unknown. The objective of this study was to determine the role of XB130 in lipopolysaccharide (LPS)-induced septic responses and acute lung injury. LPS was intr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041914/ https://www.ncbi.nlm.nih.gov/pubmed/27029000 http://dx.doi.org/10.18632/oncotarget.8326 |
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author | Toba, Hiroaki Tomankova, Tereza Wang, Yingchun Bai, Xiaohui Cho, Hae-Ra Guan, Zhehong Adeyi, Oyedele A. Tian, Feng Keshavjee, Shaf Liu, Mingyao |
author_facet | Toba, Hiroaki Tomankova, Tereza Wang, Yingchun Bai, Xiaohui Cho, Hae-Ra Guan, Zhehong Adeyi, Oyedele A. Tian, Feng Keshavjee, Shaf Liu, Mingyao |
author_sort | Toba, Hiroaki |
collection | PubMed |
description | XB130 is a novel oncoprotein that promotes cancer cell survival, proliferation and migration. Its physiological function in vivo is largely unknown. The objective of this study was to determine the role of XB130 in lipopolysaccharide (LPS)-induced septic responses and acute lung injury. LPS was intraperitoneally administrated to Xb130 knockout (KO) and wild type (WT) mice. There was a significant weight loss in KO mice at Day 2 and significantly higher disease scores during the 7 days of observation. The levels of tumor necrosis factor-alpha, monocyte chemoattractant protein-1, interleukin-6 and interleukin-10 in the serum were significantly higher in KO mice at Day 2. In KO mice there were a significantly higher lung injury score, higher wet/dry lung weight ratio, more apoptotic cells and less proliferative cells in the lung. Macrophage infiltration was significantly elevated in the lung of KO mice. There was significantly increased number of p-GSK-3β positive cells in KO mice, which were mainly neutrophils and macrophages. XB130 is expressed in alveolar type I and type II cells in the lung. The expression in these cells was significantly reduced after LPS challenge. XB130 deficiency delayed the recovery from systemic septic responses, and the presence of XB130 in the alveolar epithelial cells may provide protective mechanisms by reducing cell death and promoting cell proliferation, and reducing pulmonary permeability. |
format | Online Article Text |
id | pubmed-5041914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-50419142016-10-10 XB130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury Toba, Hiroaki Tomankova, Tereza Wang, Yingchun Bai, Xiaohui Cho, Hae-Ra Guan, Zhehong Adeyi, Oyedele A. Tian, Feng Keshavjee, Shaf Liu, Mingyao Oncotarget Research Paper XB130 is a novel oncoprotein that promotes cancer cell survival, proliferation and migration. Its physiological function in vivo is largely unknown. The objective of this study was to determine the role of XB130 in lipopolysaccharide (LPS)-induced septic responses and acute lung injury. LPS was intraperitoneally administrated to Xb130 knockout (KO) and wild type (WT) mice. There was a significant weight loss in KO mice at Day 2 and significantly higher disease scores during the 7 days of observation. The levels of tumor necrosis factor-alpha, monocyte chemoattractant protein-1, interleukin-6 and interleukin-10 in the serum were significantly higher in KO mice at Day 2. In KO mice there were a significantly higher lung injury score, higher wet/dry lung weight ratio, more apoptotic cells and less proliferative cells in the lung. Macrophage infiltration was significantly elevated in the lung of KO mice. There was significantly increased number of p-GSK-3β positive cells in KO mice, which were mainly neutrophils and macrophages. XB130 is expressed in alveolar type I and type II cells in the lung. The expression in these cells was significantly reduced after LPS challenge. XB130 deficiency delayed the recovery from systemic septic responses, and the presence of XB130 in the alveolar epithelial cells may provide protective mechanisms by reducing cell death and promoting cell proliferation, and reducing pulmonary permeability. Impact Journals LLC 2016-03-23 /pmc/articles/PMC5041914/ /pubmed/27029000 http://dx.doi.org/10.18632/oncotarget.8326 Text en Copyright: © 2016 Toba et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Toba, Hiroaki Tomankova, Tereza Wang, Yingchun Bai, Xiaohui Cho, Hae-Ra Guan, Zhehong Adeyi, Oyedele A. Tian, Feng Keshavjee, Shaf Liu, Mingyao XB130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury |
title | XB130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury |
title_full | XB130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury |
title_fullStr | XB130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury |
title_full_unstemmed | XB130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury |
title_short | XB130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury |
title_sort | xb130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041914/ https://www.ncbi.nlm.nih.gov/pubmed/27029000 http://dx.doi.org/10.18632/oncotarget.8326 |
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