Cargando…
EFEMP1 is repressed by estrogen and inhibits the epithelial-mesenchymal transition via Wnt/β-catenin signaling in endometrial carcinoma
Epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) acted as a tumor suppressor in endometrial carcinoma (EC). However, the correlation between EFEMP1 and estrogen is unknown. Here, we reported that the expression of EFEMP1 was conversely associated with ERα in en...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041938/ https://www.ncbi.nlm.nih.gov/pubmed/27015552 http://dx.doi.org/10.18632/oncotarget.8263 |
_version_ | 1782456512475561984 |
---|---|
author | Yang, Tingting Zhang, Huilin Qiu, Haifeng Li, Bilan Wang, Jingyun Du, Guiqiang Ren, Chune Wan, Xiaoping |
author_facet | Yang, Tingting Zhang, Huilin Qiu, Haifeng Li, Bilan Wang, Jingyun Du, Guiqiang Ren, Chune Wan, Xiaoping |
author_sort | Yang, Tingting |
collection | PubMed |
description | Epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) acted as a tumor suppressor in endometrial carcinoma (EC). However, the correlation between EFEMP1 and estrogen is unknown. Here, we reported that the expression of EFEMP1 was conversely associated with ERα in endometrial carcinoma tissues. In endometrial carcinoma cells, estrogen/ERα signaling significantly suppressed the expression of EFEMP1. Moreover, chromatin immunoprecipitation (CHIP) and dual-luciferase reporter assays demonstrate that estrogen/ERα bound to the estrogen response element (ERE) located in EFEMP1 promoter and repressed its expression. Besides, in vitro and in vivo, EFEMP1 could remarkably suppress the expression of epithelial-mesenchymal transition (EMT) markers such as Vimentin, Snail and the Wnt/β-catenin target genes like Cyclin-D1 and c-Myc, which could be restored when EFEMP1 was silenced. In addition, XAV93920 (the inhibitor of the Wnt/β-catenin pathway) blocked and LiCl (the activator of the Wnt/β-catenin pathway) enhanced the effect of EFEMP1 on EMT. In conclusion, we demonstrated that estrogen/ERα signal suppresses EFEMP1. Besides, EFEMP1 inhibits EMT via interfering the Wnt/β-catenin signaling. |
format | Online Article Text |
id | pubmed-5041938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-50419382016-10-10 EFEMP1 is repressed by estrogen and inhibits the epithelial-mesenchymal transition via Wnt/β-catenin signaling in endometrial carcinoma Yang, Tingting Zhang, Huilin Qiu, Haifeng Li, Bilan Wang, Jingyun Du, Guiqiang Ren, Chune Wan, Xiaoping Oncotarget Research Paper Epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) acted as a tumor suppressor in endometrial carcinoma (EC). However, the correlation between EFEMP1 and estrogen is unknown. Here, we reported that the expression of EFEMP1 was conversely associated with ERα in endometrial carcinoma tissues. In endometrial carcinoma cells, estrogen/ERα signaling significantly suppressed the expression of EFEMP1. Moreover, chromatin immunoprecipitation (CHIP) and dual-luciferase reporter assays demonstrate that estrogen/ERα bound to the estrogen response element (ERE) located in EFEMP1 promoter and repressed its expression. Besides, in vitro and in vivo, EFEMP1 could remarkably suppress the expression of epithelial-mesenchymal transition (EMT) markers such as Vimentin, Snail and the Wnt/β-catenin target genes like Cyclin-D1 and c-Myc, which could be restored when EFEMP1 was silenced. In addition, XAV93920 (the inhibitor of the Wnt/β-catenin pathway) blocked and LiCl (the activator of the Wnt/β-catenin pathway) enhanced the effect of EFEMP1 on EMT. In conclusion, we demonstrated that estrogen/ERα signal suppresses EFEMP1. Besides, EFEMP1 inhibits EMT via interfering the Wnt/β-catenin signaling. Impact Journals LLC 2016-03-22 /pmc/articles/PMC5041938/ /pubmed/27015552 http://dx.doi.org/10.18632/oncotarget.8263 Text en Copyright: © 2016 Yang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Yang, Tingting Zhang, Huilin Qiu, Haifeng Li, Bilan Wang, Jingyun Du, Guiqiang Ren, Chune Wan, Xiaoping EFEMP1 is repressed by estrogen and inhibits the epithelial-mesenchymal transition via Wnt/β-catenin signaling in endometrial carcinoma |
title | EFEMP1 is repressed by estrogen and inhibits the epithelial-mesenchymal transition via Wnt/β-catenin signaling in endometrial carcinoma |
title_full | EFEMP1 is repressed by estrogen and inhibits the epithelial-mesenchymal transition via Wnt/β-catenin signaling in endometrial carcinoma |
title_fullStr | EFEMP1 is repressed by estrogen and inhibits the epithelial-mesenchymal transition via Wnt/β-catenin signaling in endometrial carcinoma |
title_full_unstemmed | EFEMP1 is repressed by estrogen and inhibits the epithelial-mesenchymal transition via Wnt/β-catenin signaling in endometrial carcinoma |
title_short | EFEMP1 is repressed by estrogen and inhibits the epithelial-mesenchymal transition via Wnt/β-catenin signaling in endometrial carcinoma |
title_sort | efemp1 is repressed by estrogen and inhibits the epithelial-mesenchymal transition via wnt/β-catenin signaling in endometrial carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041938/ https://www.ncbi.nlm.nih.gov/pubmed/27015552 http://dx.doi.org/10.18632/oncotarget.8263 |
work_keys_str_mv | AT yangtingting efemp1isrepressedbyestrogenandinhibitstheepithelialmesenchymaltransitionviawntbcateninsignalinginendometrialcarcinoma AT zhanghuilin efemp1isrepressedbyestrogenandinhibitstheepithelialmesenchymaltransitionviawntbcateninsignalinginendometrialcarcinoma AT qiuhaifeng efemp1isrepressedbyestrogenandinhibitstheepithelialmesenchymaltransitionviawntbcateninsignalinginendometrialcarcinoma AT libilan efemp1isrepressedbyestrogenandinhibitstheepithelialmesenchymaltransitionviawntbcateninsignalinginendometrialcarcinoma AT wangjingyun efemp1isrepressedbyestrogenandinhibitstheepithelialmesenchymaltransitionviawntbcateninsignalinginendometrialcarcinoma AT duguiqiang efemp1isrepressedbyestrogenandinhibitstheepithelialmesenchymaltransitionviawntbcateninsignalinginendometrialcarcinoma AT renchune efemp1isrepressedbyestrogenandinhibitstheepithelialmesenchymaltransitionviawntbcateninsignalinginendometrialcarcinoma AT wanxiaoping efemp1isrepressedbyestrogenandinhibitstheepithelialmesenchymaltransitionviawntbcateninsignalinginendometrialcarcinoma |