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LIM domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of Interleukin-11

Mechanisms of stromal-epithelial crosstalk are essential for Prostate cancer (PCa) tumorigenesis and progression. Peripheral zone of the prostate gland possesses a stronger inclination for PCa than transition zone. We previously found a variety of genes that differently expressed among different pro...

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Autores principales: Jiang, Chen-Yi, Yu, Jun-Jie, Ruan, Yuan, Wang, Xiao-Hai, Zhao, Wei, Wang, Xing-Jie, Zhu, Yi-Ping, Gao, Yuan, Hao, Kui-Yuan, Chen, Lei, Han, Bang-Min, Xia, Shu-Jie, Zhao, Fu-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041978/
https://www.ncbi.nlm.nih.gov/pubmed/27028859
http://dx.doi.org/10.18632/oncotarget.8359
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author Jiang, Chen-Yi
Yu, Jun-Jie
Ruan, Yuan
Wang, Xiao-Hai
Zhao, Wei
Wang, Xing-Jie
Zhu, Yi-Ping
Gao, Yuan
Hao, Kui-Yuan
Chen, Lei
Han, Bang-Min
Xia, Shu-Jie
Zhao, Fu-Jun
author_facet Jiang, Chen-Yi
Yu, Jun-Jie
Ruan, Yuan
Wang, Xiao-Hai
Zhao, Wei
Wang, Xing-Jie
Zhu, Yi-Ping
Gao, Yuan
Hao, Kui-Yuan
Chen, Lei
Han, Bang-Min
Xia, Shu-Jie
Zhao, Fu-Jun
author_sort Jiang, Chen-Yi
collection PubMed
description Mechanisms of stromal-epithelial crosstalk are essential for Prostate cancer (PCa) tumorigenesis and progression. Peripheral zone of the prostate gland possesses a stronger inclination for PCa than transition zone. We previously found a variety of genes that differently expressed among different prostate stromal cells, including LIM domain only 2 (LMO2) which highly expressed in peripheral zone derived stromal cells (PZSCs) and PCa associated fibroblasts (CAFs) compared to transition zone derived stromal cells (TZSCs). Studies on its role in tumors have highlighted LMO2 as an oncogene. Herein, we aim to study the potential mechanisms of stromal LMO2 in promoting PCa progression. The in vitro cells co-culture and in vivo cells recombination revealed that LMO2 over-expressed prostate stromal cells could promote the proliferation and invasiveness of either prostate epithelial or cancer cells. Further protein array screening confirmed that stromal LMO2 stimulated the secretion of Interleukin-11 (IL-11), which could promote proliferation and invasiveness of PCa cells via IL-11 receptor α (IL11Rα) – STAT3 signaling. Moreover, stromal LMO2 over-expression could suppress miR-204-5p which was proven to be a negative regulator of IL-11 expression. Taken together, results of our study demonstrate that prostate stromal LMO2 is capable of stimulating IL-11 secretion and by which activates IL11Rα – STAT3 signaling in PCa cells and then facilitates PCa progression. These results may make stromal LMO2 responsible for zonal characteristic of PCa and as a target for PCa microenvironment-targeted therapy.
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spelling pubmed-50419782016-10-10 LIM domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of Interleukin-11 Jiang, Chen-Yi Yu, Jun-Jie Ruan, Yuan Wang, Xiao-Hai Zhao, Wei Wang, Xing-Jie Zhu, Yi-Ping Gao, Yuan Hao, Kui-Yuan Chen, Lei Han, Bang-Min Xia, Shu-Jie Zhao, Fu-Jun Oncotarget Research Paper Mechanisms of stromal-epithelial crosstalk are essential for Prostate cancer (PCa) tumorigenesis and progression. Peripheral zone of the prostate gland possesses a stronger inclination for PCa than transition zone. We previously found a variety of genes that differently expressed among different prostate stromal cells, including LIM domain only 2 (LMO2) which highly expressed in peripheral zone derived stromal cells (PZSCs) and PCa associated fibroblasts (CAFs) compared to transition zone derived stromal cells (TZSCs). Studies on its role in tumors have highlighted LMO2 as an oncogene. Herein, we aim to study the potential mechanisms of stromal LMO2 in promoting PCa progression. The in vitro cells co-culture and in vivo cells recombination revealed that LMO2 over-expressed prostate stromal cells could promote the proliferation and invasiveness of either prostate epithelial or cancer cells. Further protein array screening confirmed that stromal LMO2 stimulated the secretion of Interleukin-11 (IL-11), which could promote proliferation and invasiveness of PCa cells via IL-11 receptor α (IL11Rα) – STAT3 signaling. Moreover, stromal LMO2 over-expression could suppress miR-204-5p which was proven to be a negative regulator of IL-11 expression. Taken together, results of our study demonstrate that prostate stromal LMO2 is capable of stimulating IL-11 secretion and by which activates IL11Rα – STAT3 signaling in PCa cells and then facilitates PCa progression. These results may make stromal LMO2 responsible for zonal characteristic of PCa and as a target for PCa microenvironment-targeted therapy. Impact Journals LLC 2016-03-25 /pmc/articles/PMC5041978/ /pubmed/27028859 http://dx.doi.org/10.18632/oncotarget.8359 Text en Copyright: © 2016 Jiang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Jiang, Chen-Yi
Yu, Jun-Jie
Ruan, Yuan
Wang, Xiao-Hai
Zhao, Wei
Wang, Xing-Jie
Zhu, Yi-Ping
Gao, Yuan
Hao, Kui-Yuan
Chen, Lei
Han, Bang-Min
Xia, Shu-Jie
Zhao, Fu-Jun
LIM domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of Interleukin-11
title LIM domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of Interleukin-11
title_full LIM domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of Interleukin-11
title_fullStr LIM domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of Interleukin-11
title_full_unstemmed LIM domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of Interleukin-11
title_short LIM domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of Interleukin-11
title_sort lim domain only 2 over-expression in prostate stromal cells facilitates prostate cancer progression through paracrine of interleukin-11
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5041978/
https://www.ncbi.nlm.nih.gov/pubmed/27028859
http://dx.doi.org/10.18632/oncotarget.8359
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