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Vascular proteomics in metabolic and cardiovascular diseases

The vasculature is essential for proper organ function. Many pathologies are directly and indirectly related to vascular dysfunction, which causes significant morbidity and mortality. A common pathophysiological feature of diseased vessels is extracellular matrix (ECM) remodelling. Analysing the pro...

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Autores principales: Lynch, M., Barallobre‐Barreiro, J., Jahangiri, M., Mayr, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042041/
https://www.ncbi.nlm.nih.gov/pubmed/26940365
http://dx.doi.org/10.1111/joim.12486
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author Lynch, M.
Barallobre‐Barreiro, J.
Jahangiri, M.
Mayr, M.
author_facet Lynch, M.
Barallobre‐Barreiro, J.
Jahangiri, M.
Mayr, M.
author_sort Lynch, M.
collection PubMed
description The vasculature is essential for proper organ function. Many pathologies are directly and indirectly related to vascular dysfunction, which causes significant morbidity and mortality. A common pathophysiological feature of diseased vessels is extracellular matrix (ECM) remodelling. Analysing the protein composition of the ECM by conventional antibody‐based techniques is challenging; alternative splicing or post‐translational modifications, such as glycosylation, can mask epitopes required for antibody recognition. By contrast, proteomic analysis by mass spectrometry enables the study of proteins without the constraints of antibodies. Recent advances in proteomic techniques make it feasible to characterize the composition of the vascular ECM and its remodelling in disease. These developments may lead to the discovery of novel prognostic and diagnostic markers. Thus, proteomics holds potential for identifying ECM signatures to monitor vascular disease processes. Furthermore, a better understanding of the ECM remodelling processes in the vasculature might make ECM‐associated proteins more attractive targets for drug discovery efforts. In this review, we will summarize the role of the ECM in the vasculature. Then, we will describe the challenges associated with studying the intricate network of ECM proteins and the current proteomic strategies to analyse the vascular ECM in metabolic and cardiovascular diseases.
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spelling pubmed-50420412016-10-03 Vascular proteomics in metabolic and cardiovascular diseases Lynch, M. Barallobre‐Barreiro, J. Jahangiri, M. Mayr, M. J Intern Med Review Articles The vasculature is essential for proper organ function. Many pathologies are directly and indirectly related to vascular dysfunction, which causes significant morbidity and mortality. A common pathophysiological feature of diseased vessels is extracellular matrix (ECM) remodelling. Analysing the protein composition of the ECM by conventional antibody‐based techniques is challenging; alternative splicing or post‐translational modifications, such as glycosylation, can mask epitopes required for antibody recognition. By contrast, proteomic analysis by mass spectrometry enables the study of proteins without the constraints of antibodies. Recent advances in proteomic techniques make it feasible to characterize the composition of the vascular ECM and its remodelling in disease. These developments may lead to the discovery of novel prognostic and diagnostic markers. Thus, proteomics holds potential for identifying ECM signatures to monitor vascular disease processes. Furthermore, a better understanding of the ECM remodelling processes in the vasculature might make ECM‐associated proteins more attractive targets for drug discovery efforts. In this review, we will summarize the role of the ECM in the vasculature. Then, we will describe the challenges associated with studying the intricate network of ECM proteins and the current proteomic strategies to analyse the vascular ECM in metabolic and cardiovascular diseases. John Wiley and Sons Inc. 2016-03-04 2016-10 /pmc/articles/PMC5042041/ /pubmed/26940365 http://dx.doi.org/10.1111/joim.12486 Text en © 2016 The Authors. Journal of Internal Medicine published by John Wiley & Sons Ltd on behalf of Association for Publication of The Journal of Internal Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Articles
Lynch, M.
Barallobre‐Barreiro, J.
Jahangiri, M.
Mayr, M.
Vascular proteomics in metabolic and cardiovascular diseases
title Vascular proteomics in metabolic and cardiovascular diseases
title_full Vascular proteomics in metabolic and cardiovascular diseases
title_fullStr Vascular proteomics in metabolic and cardiovascular diseases
title_full_unstemmed Vascular proteomics in metabolic and cardiovascular diseases
title_short Vascular proteomics in metabolic and cardiovascular diseases
title_sort vascular proteomics in metabolic and cardiovascular diseases
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042041/
https://www.ncbi.nlm.nih.gov/pubmed/26940365
http://dx.doi.org/10.1111/joim.12486
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