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Flexible and rapid construction of viral chimeras applied to hepatitis C virus

A novel and broadly applicable strategy combining site-directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using hepatitis C virus (HCV) as an exemplar. Full-length HCV genomic cloning cassettes, which contained flexibly situated restriction endonuclease sites...

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Detalles Bibliográficos
Autores principales:	McClure, C. Patrick, Urbanowicz, Richard A., King, Barnabas J., Cano-Crespo, Sara, Tarr, Alexander W., Ball, Jonathan K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Microbiology Society 2016
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042125/ https://www.ncbi.nlm.nih.gov/pubmed/27329374 http://dx.doi.org/10.1099/jgv.0.000530

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