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Intracellular ice formation in mouse zygotes and early morulae vs. cooling rate and temperature-experimental vs. theory
In this study, mature female mice of the ICR strain were induced to superovultate, mated, and collected at either zygote or early morula stages. Embryos suspended in 1 M ethylene glycol in PBS containing 10 mg/L Snomax for 15 min, then transferred in sample holder to Linkam cryostage, cooled to and...
Autores principales: | Jin, Bo, Seki, Shinsuke, Paredes, Estefania, Qiu, Juan, Shi, Yanbin, Zhang, Zhenqiang, Ma, Chao, Jiang, Shuyan, Li, Jiaqi, Yuan, Feng, Wang, Shu, Shao, Xiaoguang, Mazur, Peter |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042431/ https://www.ncbi.nlm.nih.gov/pubmed/27481511 http://dx.doi.org/10.1016/j.cryobiol.2016.07.014 |
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