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Quantitative imaging reveals real-time Pou5f3–Nanog complexes driving dorsoventral mesendoderm patterning in zebrafish

Formation of the three embryonic germ layers is a fundamental developmental process that initiates differentiation. How the zebrafish pluripotency factor Pou5f3 (homologous to mammalian Oct4) drives lineage commitment is unclear. Here, we introduce fluorescence lifetime imaging microscopy and fluore...

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Detalles Bibliográficos
Autores principales:	Perez-Camps, Mireia, Tian, Jing, Chng, Serene C, Sem, Kai Pin, Sudhaharan, Thankiah, Teh, Cathleen, Wachsmuth, Malte, Korzh, Vladimir, Ahmed, Sohail, Reversade, Bruno
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2016
Materias:	Biophysics and Structural Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042653/ https://www.ncbi.nlm.nih.gov/pubmed/27684073 http://dx.doi.org/10.7554/eLife.11475

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