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Differential activation of c-Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats

Proto-oncogene c-Fos (c-Fos) is frequently used to detect a pathogenesis in central nervous system disorders. The present study examined changes in the immunoreactivity of c-Fos in the paraventricular nucleus of the hypothalamus (PVNH) and paraventricular nucleus of the thalamus (PVNT) following myo...

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Autores principales: Tae, Hyun-Jin, Park, Seung Min, Cho, Jeong Hwi, Kim, In Hye, Ahn, Ji Hyeon, Park, Joon Ha, Won, Moo-Ho, Chen, Bai Hui, Shin, Bich-Na, Shin, Myoung Cheol, Lee, Choong Hyun, Hong, Seongkweon, Lee, Jae-Chul, Cho, Jun Hwi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042731/
https://www.ncbi.nlm.nih.gov/pubmed/27601012
http://dx.doi.org/10.3892/mmr.2016.5731
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author Tae, Hyun-Jin
Park, Seung Min
Cho, Jeong Hwi
Kim, In Hye
Ahn, Ji Hyeon
Park, Joon Ha
Won, Moo-Ho
Chen, Bai Hui
Shin, Bich-Na
Shin, Myoung Cheol
Lee, Choong Hyun
Hong, Seongkweon
Lee, Jae-Chul
Cho, Jun Hwi
author_facet Tae, Hyun-Jin
Park, Seung Min
Cho, Jeong Hwi
Kim, In Hye
Ahn, Ji Hyeon
Park, Joon Ha
Won, Moo-Ho
Chen, Bai Hui
Shin, Bich-Na
Shin, Myoung Cheol
Lee, Choong Hyun
Hong, Seongkweon
Lee, Jae-Chul
Cho, Jun Hwi
author_sort Tae, Hyun-Jin
collection PubMed
description Proto-oncogene c-Fos (c-Fos) is frequently used to detect a pathogenesis in central nervous system disorders. The present study examined changes in the immunoreactivity of c-Fos in the paraventricular nucleus of the hypothalamus (PVNH) and paraventricular nucleus of the thalamus (PVNT) following myocardial infarction (MI) in rats. Infarction in the left ventricle was examined by Masson's trichrome staining. Neuronal degeneration was monitored for 56 days after MI using crystal violet and Fluoro-Jade B histofluorescence staining. Changes in the immunoreactivity of c-Fos were determined using immunohistochemistry for c-Fos. The average infarct size of the left ventricle circumference was ~44% subsequent to MI. Neuronal degeneration was not detected in PVNH and PVNT following MI. c-Fos immunoreactive ((+)) cells were infrequently observed in the nuclei of the sham-group. However, the number of c-Fos(+) cells was increased in the nuclei following MI and peaked in the PVNH and PVNT at 3 and 14 days, respectively. The number of c-Fos(+) cells were comparable with the sham group at 56 days after MI. Therefore, MI may induce c-Fos immunoreactivity in PVNH and PVNT, this increase of c-Fos expression levels may be associated with the stress that occurs in the brain following MI.
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spelling pubmed-50427312016-10-05 Differential activation of c-Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats Tae, Hyun-Jin Park, Seung Min Cho, Jeong Hwi Kim, In Hye Ahn, Ji Hyeon Park, Joon Ha Won, Moo-Ho Chen, Bai Hui Shin, Bich-Na Shin, Myoung Cheol Lee, Choong Hyun Hong, Seongkweon Lee, Jae-Chul Cho, Jun Hwi Mol Med Rep Articles Proto-oncogene c-Fos (c-Fos) is frequently used to detect a pathogenesis in central nervous system disorders. The present study examined changes in the immunoreactivity of c-Fos in the paraventricular nucleus of the hypothalamus (PVNH) and paraventricular nucleus of the thalamus (PVNT) following myocardial infarction (MI) in rats. Infarction in the left ventricle was examined by Masson's trichrome staining. Neuronal degeneration was monitored for 56 days after MI using crystal violet and Fluoro-Jade B histofluorescence staining. Changes in the immunoreactivity of c-Fos were determined using immunohistochemistry for c-Fos. The average infarct size of the left ventricle circumference was ~44% subsequent to MI. Neuronal degeneration was not detected in PVNH and PVNT following MI. c-Fos immunoreactive ((+)) cells were infrequently observed in the nuclei of the sham-group. However, the number of c-Fos(+) cells was increased in the nuclei following MI and peaked in the PVNH and PVNT at 3 and 14 days, respectively. The number of c-Fos(+) cells were comparable with the sham group at 56 days after MI. Therefore, MI may induce c-Fos immunoreactivity in PVNH and PVNT, this increase of c-Fos expression levels may be associated with the stress that occurs in the brain following MI. D.A. Spandidos 2016-10 2016-09-07 /pmc/articles/PMC5042731/ /pubmed/27601012 http://dx.doi.org/10.3892/mmr.2016.5731 Text en Copyright: © Tae et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Tae, Hyun-Jin
Park, Seung Min
Cho, Jeong Hwi
Kim, In Hye
Ahn, Ji Hyeon
Park, Joon Ha
Won, Moo-Ho
Chen, Bai Hui
Shin, Bich-Na
Shin, Myoung Cheol
Lee, Choong Hyun
Hong, Seongkweon
Lee, Jae-Chul
Cho, Jun Hwi
Differential activation of c-Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats
title Differential activation of c-Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats
title_full Differential activation of c-Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats
title_fullStr Differential activation of c-Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats
title_full_unstemmed Differential activation of c-Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats
title_short Differential activation of c-Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats
title_sort differential activation of c-fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042731/
https://www.ncbi.nlm.nih.gov/pubmed/27601012
http://dx.doi.org/10.3892/mmr.2016.5731
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