Cargando…
Human tonsil-derived mesenchymal stromal cells enhanced myelopoiesis in a mouse model of allogeneic bone marrow transplantation
Mesenchymal stromal cells (MSCs) have therapeutic potential for repairing tissue damage and are involved in immune regulation. MSCs are predominantly isolated from bone marrow (BM), adipose tissue or placental tissue. Further to these well-known sources, the isolation of MSCs from human tonsils was...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042779/ https://www.ncbi.nlm.nih.gov/pubmed/27511380 http://dx.doi.org/10.3892/mmr.2016.5604 |
_version_ | 1782456649459433472 |
---|---|
author | Ryu, Jung-Hwa Park, Minhwa Kim, Bo-Kyung Kim, Yu-Hee Woo, So-Youn Ryu, Kyung-Ha |
author_facet | Ryu, Jung-Hwa Park, Minhwa Kim, Bo-Kyung Kim, Yu-Hee Woo, So-Youn Ryu, Kyung-Ha |
author_sort | Ryu, Jung-Hwa |
collection | PubMed |
description | Mesenchymal stromal cells (MSCs) have therapeutic potential for repairing tissue damage and are involved in immune regulation. MSCs are predominantly isolated from bone marrow (BM), adipose tissue or placental tissue. Further to these well-known sources, the isolation of MSCs from human tonsils was previously reported. The aim of the present study was to investigate a potential role for tonsil-derived MSCs (T-MSCs) in BM reconstitution and application towards supplementing hematopoiesis in a mouse model of BM transplantation (BMT). Eight-week-old BALB/c female mice received 80 mg/kg busulfan (Bu)/200 mg/kg cyclophosphamide (Cy) conditioning chemotherapy for BM ablation. Subsequently, human T-MSCs were injected into the Bu/Cy-treated mice with or without BM cells (BMCs) obtained from allogeneic C57BL/6 male mice. After 3 weeks, peripheral blood and BM was collected for analysis. The red blood cell count in the group that received BMCs had almost returned to normal, whereas mononuclear cell counts and BM cellularity were most improved in the T-MSCs + BMCs group. These results indicate that the T-MSCs enhanced myelopoiesis in the allogeneic BMT mouse model, as evidenced by the restoration of BM with hematopoietic cells, as well as increased myeloid colony formation in vitro. Therefore, T-MSCs may provide a source of MSCs to facilitate myelopoiesis and megakaryocytosis following BMT. |
format | Online Article Text |
id | pubmed-5042779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-50427792016-10-05 Human tonsil-derived mesenchymal stromal cells enhanced myelopoiesis in a mouse model of allogeneic bone marrow transplantation Ryu, Jung-Hwa Park, Minhwa Kim, Bo-Kyung Kim, Yu-Hee Woo, So-Youn Ryu, Kyung-Ha Mol Med Rep Articles Mesenchymal stromal cells (MSCs) have therapeutic potential for repairing tissue damage and are involved in immune regulation. MSCs are predominantly isolated from bone marrow (BM), adipose tissue or placental tissue. Further to these well-known sources, the isolation of MSCs from human tonsils was previously reported. The aim of the present study was to investigate a potential role for tonsil-derived MSCs (T-MSCs) in BM reconstitution and application towards supplementing hematopoiesis in a mouse model of BM transplantation (BMT). Eight-week-old BALB/c female mice received 80 mg/kg busulfan (Bu)/200 mg/kg cyclophosphamide (Cy) conditioning chemotherapy for BM ablation. Subsequently, human T-MSCs were injected into the Bu/Cy-treated mice with or without BM cells (BMCs) obtained from allogeneic C57BL/6 male mice. After 3 weeks, peripheral blood and BM was collected for analysis. The red blood cell count in the group that received BMCs had almost returned to normal, whereas mononuclear cell counts and BM cellularity were most improved in the T-MSCs + BMCs group. These results indicate that the T-MSCs enhanced myelopoiesis in the allogeneic BMT mouse model, as evidenced by the restoration of BM with hematopoietic cells, as well as increased myeloid colony formation in vitro. Therefore, T-MSCs may provide a source of MSCs to facilitate myelopoiesis and megakaryocytosis following BMT. D.A. Spandidos 2016-10 2016-08-08 /pmc/articles/PMC5042779/ /pubmed/27511380 http://dx.doi.org/10.3892/mmr.2016.5604 Text en Copyright: © Ryu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Ryu, Jung-Hwa Park, Minhwa Kim, Bo-Kyung Kim, Yu-Hee Woo, So-Youn Ryu, Kyung-Ha Human tonsil-derived mesenchymal stromal cells enhanced myelopoiesis in a mouse model of allogeneic bone marrow transplantation |
title | Human tonsil-derived mesenchymal stromal cells enhanced myelopoiesis in a mouse model of allogeneic bone marrow transplantation |
title_full | Human tonsil-derived mesenchymal stromal cells enhanced myelopoiesis in a mouse model of allogeneic bone marrow transplantation |
title_fullStr | Human tonsil-derived mesenchymal stromal cells enhanced myelopoiesis in a mouse model of allogeneic bone marrow transplantation |
title_full_unstemmed | Human tonsil-derived mesenchymal stromal cells enhanced myelopoiesis in a mouse model of allogeneic bone marrow transplantation |
title_short | Human tonsil-derived mesenchymal stromal cells enhanced myelopoiesis in a mouse model of allogeneic bone marrow transplantation |
title_sort | human tonsil-derived mesenchymal stromal cells enhanced myelopoiesis in a mouse model of allogeneic bone marrow transplantation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042779/ https://www.ncbi.nlm.nih.gov/pubmed/27511380 http://dx.doi.org/10.3892/mmr.2016.5604 |
work_keys_str_mv | AT ryujunghwa humantonsilderivedmesenchymalstromalcellsenhancedmyelopoiesisinamousemodelofallogeneicbonemarrowtransplantation AT parkminhwa humantonsilderivedmesenchymalstromalcellsenhancedmyelopoiesisinamousemodelofallogeneicbonemarrowtransplantation AT kimbokyung humantonsilderivedmesenchymalstromalcellsenhancedmyelopoiesisinamousemodelofallogeneicbonemarrowtransplantation AT kimyuhee humantonsilderivedmesenchymalstromalcellsenhancedmyelopoiesisinamousemodelofallogeneicbonemarrowtransplantation AT woosoyoun humantonsilderivedmesenchymalstromalcellsenhancedmyelopoiesisinamousemodelofallogeneicbonemarrowtransplantation AT ryukyungha humantonsilderivedmesenchymalstromalcellsenhancedmyelopoiesisinamousemodelofallogeneicbonemarrowtransplantation |