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Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile
T cells are known to be plastic and to change their phenotype according to the cellular and biochemical milieu they are embedded in. In this study, we transposed this concept at a macroscopic level assessing whether changes in the environmental housing conditions of C57/BL6 mice would influence the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042968/ https://www.ncbi.nlm.nih.gov/pubmed/27746779 http://dx.doi.org/10.3389/fimmu.2016.00381 |
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author | Rattazzi, Lorenza Piras, Giuseppa Brod, Samuel Smith, Koval Ono, Masahiro D’Acquisto, Fulvio |
author_facet | Rattazzi, Lorenza Piras, Giuseppa Brod, Samuel Smith, Koval Ono, Masahiro D’Acquisto, Fulvio |
author_sort | Rattazzi, Lorenza |
collection | PubMed |
description | T cells are known to be plastic and to change their phenotype according to the cellular and biochemical milieu they are embedded in. In this study, we transposed this concept at a macroscopic level assessing whether changes in the environmental housing conditions of C57/BL6 mice would influence the phenotype and function of T cells. Our study shows that exposure to 2 weeks in an enriched environment (EE) does not impact the T cell repertoire in vivo and causes no changes in the early TCR-driven activation events of these cells. Surprisingly, however, T cells from enriched mice showed a unique T helper effector cell phenotype upon differentiation in vitro. This was featured by a significant reduction in their ability to produce IFN-γ and by an increased release of IL-10 and IL-17. Microarray analysis of these cells also revealed a unique gene fingerprint with key signaling pathways involved in autoimmunity being modulated. Together, our results provide first evidence for a specific effect of EE on T cell differentiation and its associated changes in gene expression profile. In addition, our study sheds new light on the possible mechanisms by which changes in environmental factors can significantly influence the immune response of the host and favor the resolution of the inflammatory response. |
format | Online Article Text |
id | pubmed-5042968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50429682016-10-14 Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile Rattazzi, Lorenza Piras, Giuseppa Brod, Samuel Smith, Koval Ono, Masahiro D’Acquisto, Fulvio Front Immunol Immunology T cells are known to be plastic and to change their phenotype according to the cellular and biochemical milieu they are embedded in. In this study, we transposed this concept at a macroscopic level assessing whether changes in the environmental housing conditions of C57/BL6 mice would influence the phenotype and function of T cells. Our study shows that exposure to 2 weeks in an enriched environment (EE) does not impact the T cell repertoire in vivo and causes no changes in the early TCR-driven activation events of these cells. Surprisingly, however, T cells from enriched mice showed a unique T helper effector cell phenotype upon differentiation in vitro. This was featured by a significant reduction in their ability to produce IFN-γ and by an increased release of IL-10 and IL-17. Microarray analysis of these cells also revealed a unique gene fingerprint with key signaling pathways involved in autoimmunity being modulated. Together, our results provide first evidence for a specific effect of EE on T cell differentiation and its associated changes in gene expression profile. In addition, our study sheds new light on the possible mechanisms by which changes in environmental factors can significantly influence the immune response of the host and favor the resolution of the inflammatory response. Frontiers Media S.A. 2016-09-30 /pmc/articles/PMC5042968/ /pubmed/27746779 http://dx.doi.org/10.3389/fimmu.2016.00381 Text en Copyright © 2016 Rattazzi, Piras, Brod, Smith, Ono and D’Acquisto. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Rattazzi, Lorenza Piras, Giuseppa Brod, Samuel Smith, Koval Ono, Masahiro D’Acquisto, Fulvio Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile |
title | Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile |
title_full | Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile |
title_fullStr | Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile |
title_full_unstemmed | Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile |
title_short | Impact of Enriched Environment on Murine T Cell Differentiation and Gene Expression Profile |
title_sort | impact of enriched environment on murine t cell differentiation and gene expression profile |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042968/ https://www.ncbi.nlm.nih.gov/pubmed/27746779 http://dx.doi.org/10.3389/fimmu.2016.00381 |
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