Cargando…

In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation met...

Descripción completa

Detalles Bibliográficos
Autores principales: Ilczuk, Agnieszka, Jacygrad, Ewelina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042974/
https://www.ncbi.nlm.nih.gov/pubmed/27746667
http://dx.doi.org/10.1007/s11627-016-9781-6
_version_ 1782456674448048128
author Ilczuk, Agnieszka
Jacygrad, Ewelina
author_facet Ilczuk, Agnieszka
Jacygrad, Ewelina
author_sort Ilczuk, Agnieszka
collection PubMed
description Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars ‘Aurea’ and ‘Elegantissima’ were cultured on media enriched with six different sources of macronutrients. Media were supplemented with either N(6)-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L(−1) BA, 0.1 mg L(−1) NAA, and 20–30 g L(−1) sucrose. Rooting of regenerated shoots was achieved by an in vitro method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L(−1) NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for ‘Aurea’ and 90% for ‘Elegantissima’. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymorphic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197–199 and 184–187 distinct and reproducible band classes, respectively, in ‘Aurea’ and ‘Elegantissima’ plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant.
format Online
Article
Text
id pubmed-5042974
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-50429742016-10-14 In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers Ilczuk, Agnieszka Jacygrad, Ewelina In Vitro Cell Dev Biol Plant Micropropagation Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars ‘Aurea’ and ‘Elegantissima’ were cultured on media enriched with six different sources of macronutrients. Media were supplemented with either N(6)-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L(−1) BA, 0.1 mg L(−1) NAA, and 20–30 g L(−1) sucrose. Rooting of regenerated shoots was achieved by an in vitro method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L(−1) NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for ‘Aurea’ and 90% for ‘Elegantissima’. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymorphic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197–199 and 184–187 distinct and reproducible band classes, respectively, in ‘Aurea’ and ‘Elegantissima’ plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant. Springer US 2016-08-26 2016 /pmc/articles/PMC5042974/ /pubmed/27746667 http://dx.doi.org/10.1007/s11627-016-9781-6 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Micropropagation
Ilczuk, Agnieszka
Jacygrad, Ewelina
In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers
title In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers
title_full In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers
title_fullStr In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers
title_full_unstemmed In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers
title_short In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers
title_sort in vitro propagation and assessment of genetic stability of acclimated plantlets of cornus alba l. using rapd and issr markers
topic Micropropagation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042974/
https://www.ncbi.nlm.nih.gov/pubmed/27746667
http://dx.doi.org/10.1007/s11627-016-9781-6
work_keys_str_mv AT ilczukagnieszka invitropropagationandassessmentofgeneticstabilityofacclimatedplantletsofcornusalbalusingrapdandissrmarkers
AT jacygradewelina invitropropagationandassessmentofgeneticstabilityofacclimatedplantletsofcornusalbalusingrapdandissrmarkers