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Low Agrobacterium tumefaciens inoculum levels and a long co-culture period lead to reduced plant defense responses and increase transgenic shoot production of sunflower (Helianthus annuus L.)
Agrobacterium-mediated plant transformation is typically conducted by inoculating plant tissues with an Agrobacterium suspension containing approximately 10(8)–10(9) bacteria mL(−1), followed by a 2–3-d co-culture period. Use of longer co-culture periods could potentially increase transformation eff...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042984/ https://www.ncbi.nlm.nih.gov/pubmed/27746666 http://dx.doi.org/10.1007/s11627-016-9774-5 |