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Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster in Bacillus subtilis via Red/ET recombineering

Heterologous expression of biosynthetic pathways is an important way to research and discover microbial natural products. Bacillus subtilis is a suitable host for the heterologous production of natural products from bacilli and related Firmicutes. Existing technologies for heterologous expression of...

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Detalles Bibliográficos
Autores principales:	Liu, Qingshu, Shen, Qiyao, Bian, Xiaoying, Chen, Hanna, Fu, Jun, Wang, Hailong, Lei, Ping, Guo, Zhaohui, Chen, Wu, Li, Dingjun, Zhang, Youming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043344/ https://www.ncbi.nlm.nih.gov/pubmed/27687863 http://dx.doi.org/10.1038/srep34623

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