Cargando…
Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks
BACKGROUND: Pretreated lignocellulosic biomass is considered as a suitable feedstock for the sustainable production of chemicals. However, the recalcitrant nature of cellulose often results in very cost-intensive overall production processes. A promising concept to reduce the costs is consolidated b...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043636/ https://www.ncbi.nlm.nih.gov/pubmed/27686382 http://dx.doi.org/10.1186/s12934-016-0567-7 |
_version_ | 1782456792609980416 |
---|---|
author | Antonov, Elena Wirth, Steffen Gerlach, Tim Schlembach, Ivan Rosenbaum, Miriam A. Regestein, Lars Büchs, Jochen |
author_facet | Antonov, Elena Wirth, Steffen Gerlach, Tim Schlembach, Ivan Rosenbaum, Miriam A. Regestein, Lars Büchs, Jochen |
author_sort | Antonov, Elena |
collection | PubMed |
description | BACKGROUND: Pretreated lignocellulosic biomass is considered as a suitable feedstock for the sustainable production of chemicals. However, the recalcitrant nature of cellulose often results in very cost-intensive overall production processes. A promising concept to reduce the costs is consolidated bioprocessing, which integrates in a single step cellulase production, cellulose hydrolysis, and fermentative conversion of produced sugars into a valuable product. This approach, however, requires assessing the digestibility of the applied celluloses and, thus, the released sugar amount during the fermentation. Since the released sugars are completely taken up by Trichoderma reesei Rut-C30 and the sugar consumption is stoichiometrically coupled to oxygen uptake, the respiration activity was measured to evaluate the digestibility of cellulose. RESULTS: The method was successfully tested on commercial cellulosic substrates identifying a correlation between the respiration activity and the crystallinity of the substrate. Pulse experiments with cellulose and cellulases suggested that the respiration activity of T. reesei on cellulose can be divided into two distinct phases, one limited by enzyme activity and one by cellulose-binding-sites. The impact of known (cellobiose, sophorose, urea, tween 80, peptone) and new (miscanthus steepwater) compounds enhancing cellulase production was evaluated. Furthermore, the influence of two different pretreatment methods, the OrganoCat and OrganoSolv process, on the digestibility of beech wood saw dust was tested. CONCLUSIONS: The introduced method allows an online evaluation of cellulose digestibility in complex and non-complex cultivation media. As the measurements are performed under fermentation conditions, it is a valuable tool to test different types of cellulose for consolidated bioprocessing applications. Furthermore, the method can be applied to identify new compounds, which influence cellulase production. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-016-0567-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5043636 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-50436362016-10-05 Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks Antonov, Elena Wirth, Steffen Gerlach, Tim Schlembach, Ivan Rosenbaum, Miriam A. Regestein, Lars Büchs, Jochen Microb Cell Fact Research BACKGROUND: Pretreated lignocellulosic biomass is considered as a suitable feedstock for the sustainable production of chemicals. However, the recalcitrant nature of cellulose often results in very cost-intensive overall production processes. A promising concept to reduce the costs is consolidated bioprocessing, which integrates in a single step cellulase production, cellulose hydrolysis, and fermentative conversion of produced sugars into a valuable product. This approach, however, requires assessing the digestibility of the applied celluloses and, thus, the released sugar amount during the fermentation. Since the released sugars are completely taken up by Trichoderma reesei Rut-C30 and the sugar consumption is stoichiometrically coupled to oxygen uptake, the respiration activity was measured to evaluate the digestibility of cellulose. RESULTS: The method was successfully tested on commercial cellulosic substrates identifying a correlation between the respiration activity and the crystallinity of the substrate. Pulse experiments with cellulose and cellulases suggested that the respiration activity of T. reesei on cellulose can be divided into two distinct phases, one limited by enzyme activity and one by cellulose-binding-sites. The impact of known (cellobiose, sophorose, urea, tween 80, peptone) and new (miscanthus steepwater) compounds enhancing cellulase production was evaluated. Furthermore, the influence of two different pretreatment methods, the OrganoCat and OrganoSolv process, on the digestibility of beech wood saw dust was tested. CONCLUSIONS: The introduced method allows an online evaluation of cellulose digestibility in complex and non-complex cultivation media. As the measurements are performed under fermentation conditions, it is a valuable tool to test different types of cellulose for consolidated bioprocessing applications. Furthermore, the method can be applied to identify new compounds, which influence cellulase production. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-016-0567-7) contains supplementary material, which is available to authorized users. BioMed Central 2016-09-29 /pmc/articles/PMC5043636/ /pubmed/27686382 http://dx.doi.org/10.1186/s12934-016-0567-7 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Antonov, Elena Wirth, Steffen Gerlach, Tim Schlembach, Ivan Rosenbaum, Miriam A. Regestein, Lars Büchs, Jochen Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks |
title | Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks |
title_full | Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks |
title_fullStr | Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks |
title_full_unstemmed | Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks |
title_short | Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks |
title_sort | efficient evaluation of cellulose digestibility by trichoderma reesei rut-c30 cultures in online monitored shake flasks |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5043636/ https://www.ncbi.nlm.nih.gov/pubmed/27686382 http://dx.doi.org/10.1186/s12934-016-0567-7 |
work_keys_str_mv | AT antonovelena efficientevaluationofcellulosedigestibilitybytrichodermareeseirutc30culturesinonlinemonitoredshakeflasks AT wirthsteffen efficientevaluationofcellulosedigestibilitybytrichodermareeseirutc30culturesinonlinemonitoredshakeflasks AT gerlachtim efficientevaluationofcellulosedigestibilitybytrichodermareeseirutc30culturesinonlinemonitoredshakeflasks AT schlembachivan efficientevaluationofcellulosedigestibilitybytrichodermareeseirutc30culturesinonlinemonitoredshakeflasks AT rosenbaummiriama efficientevaluationofcellulosedigestibilitybytrichodermareeseirutc30culturesinonlinemonitoredshakeflasks AT regesteinlars efficientevaluationofcellulosedigestibilitybytrichodermareeseirutc30culturesinonlinemonitoredshakeflasks AT buchsjochen efficientevaluationofcellulosedigestibilitybytrichodermareeseirutc30culturesinonlinemonitoredshakeflasks |