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Frequently asked questions in hypoxia research
“What is the O(2) concentration in a normoxic cell culture incubator?” This and other frequently asked questions in hypoxia research will be answered in this review. Our intention is to give a simple introduction to the physics of gases that would be helpful for newcomers to the field of hypoxia res...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045069/ https://www.ncbi.nlm.nih.gov/pubmed/27774480 http://dx.doi.org/10.2147/HP.S92198 |
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author | Wenger, Roland H Kurtcuoglu, Vartan Scholz, Carsten C Marti, Hugo H Hoogewijs, David |
author_facet | Wenger, Roland H Kurtcuoglu, Vartan Scholz, Carsten C Marti, Hugo H Hoogewijs, David |
author_sort | Wenger, Roland H |
collection | PubMed |
description | “What is the O(2) concentration in a normoxic cell culture incubator?” This and other frequently asked questions in hypoxia research will be answered in this review. Our intention is to give a simple introduction to the physics of gases that would be helpful for newcomers to the field of hypoxia research. We will provide background knowledge about questions often asked, but without straightforward answers. What is O(2) concentration, and what is O(2) partial pressure? What is normoxia, and what is hypoxia? How much O(2) is experienced by a cell residing in a culture dish in vitro vs in a tissue in vivo? By the way, the O(2) concentration in a normoxic incubator is 18.6%, rather than 20.9% or 20%, as commonly stated in research publications. And this is strictly only valid for incubators at sea level. |
format | Online Article Text |
id | pubmed-5045069 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-50450692016-10-21 Frequently asked questions in hypoxia research Wenger, Roland H Kurtcuoglu, Vartan Scholz, Carsten C Marti, Hugo H Hoogewijs, David Hypoxia (Auckl) Review “What is the O(2) concentration in a normoxic cell culture incubator?” This and other frequently asked questions in hypoxia research will be answered in this review. Our intention is to give a simple introduction to the physics of gases that would be helpful for newcomers to the field of hypoxia research. We will provide background knowledge about questions often asked, but without straightforward answers. What is O(2) concentration, and what is O(2) partial pressure? What is normoxia, and what is hypoxia? How much O(2) is experienced by a cell residing in a culture dish in vitro vs in a tissue in vivo? By the way, the O(2) concentration in a normoxic incubator is 18.6%, rather than 20.9% or 20%, as commonly stated in research publications. And this is strictly only valid for incubators at sea level. Dove Medical Press 2015-09-18 /pmc/articles/PMC5045069/ /pubmed/27774480 http://dx.doi.org/10.2147/HP.S92198 Text en © 2015 Wenger et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Review Wenger, Roland H Kurtcuoglu, Vartan Scholz, Carsten C Marti, Hugo H Hoogewijs, David Frequently asked questions in hypoxia research |
title | Frequently asked questions in hypoxia research |
title_full | Frequently asked questions in hypoxia research |
title_fullStr | Frequently asked questions in hypoxia research |
title_full_unstemmed | Frequently asked questions in hypoxia research |
title_short | Frequently asked questions in hypoxia research |
title_sort | frequently asked questions in hypoxia research |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045069/ https://www.ncbi.nlm.nih.gov/pubmed/27774480 http://dx.doi.org/10.2147/HP.S92198 |
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