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Silencing homeobox C6 inhibits colorectal cancer cell proliferation

Homeobox C6 (HOXC6), a member of the homeobox family that encodes highly conserved transcription factors, plays a vital role in various carcinomas. In this study, we used a tissue microarray (TMA) consisting of 462 CRC samples to demonstrate that HOXC6 is more abundantly expressed in colorectal canc...

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Autores principales: Ji, Meiling, Feng, Qingyang, He, Guodong, Yang, Liangliang, Tang, Wentao, Lao, Xinyuan, Zhu, Dexiang, Lin, Qi, Xu, Pingping, Wei, Ye, Xu, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045391/
https://www.ncbi.nlm.nih.gov/pubmed/27081081
http://dx.doi.org/10.18632/oncotarget.8703
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author Ji, Meiling
Feng, Qingyang
He, Guodong
Yang, Liangliang
Tang, Wentao
Lao, Xinyuan
Zhu, Dexiang
Lin, Qi
Xu, Pingping
Wei, Ye
Xu, Jianmin
author_facet Ji, Meiling
Feng, Qingyang
He, Guodong
Yang, Liangliang
Tang, Wentao
Lao, Xinyuan
Zhu, Dexiang
Lin, Qi
Xu, Pingping
Wei, Ye
Xu, Jianmin
author_sort Ji, Meiling
collection PubMed
description Homeobox C6 (HOXC6), a member of the homeobox family that encodes highly conserved transcription factors, plays a vital role in various carcinomas. In this study, we used a tissue microarray (TMA) consisting of 462 CRC samples to demonstrate that HOXC6 is more abundantly expressed in colorectal cancer (CRC) tissues than adjacent normal mucosa. Clinicopathological data indicated that higher HOXC6 expression correlated with poor overall survival and was associated with primary tumor location in the right colon, primary tumor (pT) stage 3/4 and primary node (pN) stage 1/2. Multivariate analysis showed that high HOXC6 expression was an independent risk factor for poor CRC patient prognosis. HOXC6 downregulation via lentivirus-mediated expression of HOXC6-targeting shRNA reduced HCT116 cell viability and colony formation in vitro, and reduced growth of subcutaneous xenografts in nude mouse. HOXC6 thus appears to promote CRC cell proliferation and tumorigenesis through autophagy inhibition and mTOR pathway activation.
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spelling pubmed-50453912016-10-13 Silencing homeobox C6 inhibits colorectal cancer cell proliferation Ji, Meiling Feng, Qingyang He, Guodong Yang, Liangliang Tang, Wentao Lao, Xinyuan Zhu, Dexiang Lin, Qi Xu, Pingping Wei, Ye Xu, Jianmin Oncotarget Research Paper Homeobox C6 (HOXC6), a member of the homeobox family that encodes highly conserved transcription factors, plays a vital role in various carcinomas. In this study, we used a tissue microarray (TMA) consisting of 462 CRC samples to demonstrate that HOXC6 is more abundantly expressed in colorectal cancer (CRC) tissues than adjacent normal mucosa. Clinicopathological data indicated that higher HOXC6 expression correlated with poor overall survival and was associated with primary tumor location in the right colon, primary tumor (pT) stage 3/4 and primary node (pN) stage 1/2. Multivariate analysis showed that high HOXC6 expression was an independent risk factor for poor CRC patient prognosis. HOXC6 downregulation via lentivirus-mediated expression of HOXC6-targeting shRNA reduced HCT116 cell viability and colony formation in vitro, and reduced growth of subcutaneous xenografts in nude mouse. HOXC6 thus appears to promote CRC cell proliferation and tumorigenesis through autophagy inhibition and mTOR pathway activation. Impact Journals LLC 2016-04-12 /pmc/articles/PMC5045391/ /pubmed/27081081 http://dx.doi.org/10.18632/oncotarget.8703 Text en Copyright: © 2016 Ji et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Ji, Meiling
Feng, Qingyang
He, Guodong
Yang, Liangliang
Tang, Wentao
Lao, Xinyuan
Zhu, Dexiang
Lin, Qi
Xu, Pingping
Wei, Ye
Xu, Jianmin
Silencing homeobox C6 inhibits colorectal cancer cell proliferation
title Silencing homeobox C6 inhibits colorectal cancer cell proliferation
title_full Silencing homeobox C6 inhibits colorectal cancer cell proliferation
title_fullStr Silencing homeobox C6 inhibits colorectal cancer cell proliferation
title_full_unstemmed Silencing homeobox C6 inhibits colorectal cancer cell proliferation
title_short Silencing homeobox C6 inhibits colorectal cancer cell proliferation
title_sort silencing homeobox c6 inhibits colorectal cancer cell proliferation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045391/
https://www.ncbi.nlm.nih.gov/pubmed/27081081
http://dx.doi.org/10.18632/oncotarget.8703
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