Cargando…

Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health

Blood based bioenergetic profiling strategies are emerging as potential reporters of systemic mitochondrial function; however, the extent to which these measures reflect the bioenergetic capacity of other tissues is not known. The premise of this work is that highly metabolically active tissues, suc...

Descripción completa

Detalles Bibliográficos
Autores principales: Tyrrell, Daniel J., Bharadwaj, Manish S., Jorgensen, Matthew J., Register, Thomas C., Molina, Anthony J.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045569/
https://www.ncbi.nlm.nih.gov/pubmed/27693859
http://dx.doi.org/10.1016/j.redox.2016.09.009
_version_ 1782457141842411520
author Tyrrell, Daniel J.
Bharadwaj, Manish S.
Jorgensen, Matthew J.
Register, Thomas C.
Molina, Anthony J.A.
author_facet Tyrrell, Daniel J.
Bharadwaj, Manish S.
Jorgensen, Matthew J.
Register, Thomas C.
Molina, Anthony J.A.
author_sort Tyrrell, Daniel J.
collection PubMed
description Blood based bioenergetic profiling strategies are emerging as potential reporters of systemic mitochondrial function; however, the extent to which these measures reflect the bioenergetic capacity of other tissues is not known. The premise of this work is that highly metabolically active tissues, such as skeletal and cardiac muscle, are susceptible to differences in systemic bioenergetic capacity. Therefore, we tested whether the respiratory capacity of blood cells, monocytes and platelets, are related to contemporaneous respirometric assessments of skeletal and cardiac muscle mitochondria. 18 female vervet/African green monkeys (Chlorocebus aethiops sabaeus) of varying age and metabolic status were examined for this study. Monocyte and platelet maximal capacity correlated with maximal oxidative phosphorylation capacity of permeabilized skeletal muscle (R=0.75, 95% confidence interval [CI]: 0.38–0.97; R=0.51, 95%CI: 0.05–0.81; respectively), isolated skeletal muscle mitochondrial respiratory control ratio (RCR; R=0.70, 95%CI: 0.35–0.89; R=0.64, 95%CI: 0.23–0.98; respectively), and isolated cardiac muscle mitochondrial RCR (R=0.55, 95%CI: 0.22–0.86; R=0.58, 95%CI: 0.22–0.85; respectively). These results suggest that blood based bioenergetic profiling may be used to report on the bioenergetic capacity of muscle tissues. Blood cell respirometry represents an attractive alternative to tissue based assessments of mitochondrial function in human studies based on ease of access and the minimal participant burden required by these measures.
format Online
Article
Text
id pubmed-5045569
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-50455692016-10-06 Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health Tyrrell, Daniel J. Bharadwaj, Manish S. Jorgensen, Matthew J. Register, Thomas C. Molina, Anthony J.A. Redox Biol Research Paper Blood based bioenergetic profiling strategies are emerging as potential reporters of systemic mitochondrial function; however, the extent to which these measures reflect the bioenergetic capacity of other tissues is not known. The premise of this work is that highly metabolically active tissues, such as skeletal and cardiac muscle, are susceptible to differences in systemic bioenergetic capacity. Therefore, we tested whether the respiratory capacity of blood cells, monocytes and platelets, are related to contemporaneous respirometric assessments of skeletal and cardiac muscle mitochondria. 18 female vervet/African green monkeys (Chlorocebus aethiops sabaeus) of varying age and metabolic status were examined for this study. Monocyte and platelet maximal capacity correlated with maximal oxidative phosphorylation capacity of permeabilized skeletal muscle (R=0.75, 95% confidence interval [CI]: 0.38–0.97; R=0.51, 95%CI: 0.05–0.81; respectively), isolated skeletal muscle mitochondrial respiratory control ratio (RCR; R=0.70, 95%CI: 0.35–0.89; R=0.64, 95%CI: 0.23–0.98; respectively), and isolated cardiac muscle mitochondrial RCR (R=0.55, 95%CI: 0.22–0.86; R=0.58, 95%CI: 0.22–0.85; respectively). These results suggest that blood based bioenergetic profiling may be used to report on the bioenergetic capacity of muscle tissues. Blood cell respirometry represents an attractive alternative to tissue based assessments of mitochondrial function in human studies based on ease of access and the minimal participant burden required by these measures. Elsevier 2016-09-21 /pmc/articles/PMC5045569/ /pubmed/27693859 http://dx.doi.org/10.1016/j.redox.2016.09.009 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Tyrrell, Daniel J.
Bharadwaj, Manish S.
Jorgensen, Matthew J.
Register, Thomas C.
Molina, Anthony J.A.
Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health
title Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health
title_full Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health
title_fullStr Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health
title_full_unstemmed Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health
title_short Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health
title_sort blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: implications for a minimally invasive biomarker of mitochondrial health
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045569/
https://www.ncbi.nlm.nih.gov/pubmed/27693859
http://dx.doi.org/10.1016/j.redox.2016.09.009
work_keys_str_mv AT tyrrelldanielj bloodcellrespirometryisassociatedwithskeletalandcardiacmusclebioenergeticsimplicationsforaminimallyinvasivebiomarkerofmitochondrialhealth
AT bharadwajmanishs bloodcellrespirometryisassociatedwithskeletalandcardiacmusclebioenergeticsimplicationsforaminimallyinvasivebiomarkerofmitochondrialhealth
AT jorgensenmatthewj bloodcellrespirometryisassociatedwithskeletalandcardiacmusclebioenergeticsimplicationsforaminimallyinvasivebiomarkerofmitochondrialhealth
AT registerthomasc bloodcellrespirometryisassociatedwithskeletalandcardiacmusclebioenergeticsimplicationsforaminimallyinvasivebiomarkerofmitochondrialhealth
AT molinaanthonyja bloodcellrespirometryisassociatedwithskeletalandcardiacmusclebioenergeticsimplicationsforaminimallyinvasivebiomarkerofmitochondrialhealth