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The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats

BACKGROUND: Epidural fibrosis often causes serious complications in patients after lumbar laminectomy and discectomy and is associated with the proliferation of fibroblasts. Suramin is known to have an obvious inhibitory effect on the coactions of many growth factors and their receptors, but little...

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Autores principales: Dai, Jihang, Li, Xiaolei, Yan, Lianqi, Chen, Hui, He, Jun, Wang, Shuguang, Wang, Jingcheng, Sun, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045610/
https://www.ncbi.nlm.nih.gov/pubmed/27716371
http://dx.doi.org/10.1186/s13018-016-0443-5
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author Dai, Jihang
Li, Xiaolei
Yan, Lianqi
Chen, Hui
He, Jun
Wang, Shuguang
Wang, Jingcheng
Sun, Yu
author_facet Dai, Jihang
Li, Xiaolei
Yan, Lianqi
Chen, Hui
He, Jun
Wang, Shuguang
Wang, Jingcheng
Sun, Yu
author_sort Dai, Jihang
collection PubMed
description BACKGROUND: Epidural fibrosis often causes serious complications in patients after lumbar laminectomy and discectomy and is associated with the proliferation of fibroblasts. Suramin is known to have an obvious inhibitory effect on the coactions of many growth factors and their receptors, but little was previously known about the effect of suramin on fibroblast proliferation and the progress of epidural fibrosis. METHODS: We illustrated the effect of suramin on cultured fibroblasts of rats with different concentrations (0, 200, 400, 600 mg/l). The proliferation of suramin-treated fibroblasts was evaluated by CCK-8 and western blot analysis. Additionally, in a rat model of laminectomy, different concentrations of suramin (100, 200, and 300 mg/ml) and saline were applied to the laminectomy sites locally. The effect of suramin on preventing epidural fibrosis was detected by the Rydell classification, hydroxyproline content, histological analysis, and collagen density analyses. RESULTS: The results of CCK-8 shown that suramin could significantly inhibit fibroblasts proliferation in a dose-dependent manner. The western blotting shown that the expression levels of the cell proliferation markers such as cyclin D1, cyclin E, and PCNA were down-regulated. Moreover, in a rat model, we found that suramin could reduce epidural fibrosis as well as inhibit fibroblast proliferation, and 300 mg/ml suramin had better effect. CONCLUSIONS: Topical application of suramin could reduce epidural fibrosis after laminectomy, and the application of suramin could inhibit the proliferation of fibroblasts in rats. This study indicates that suramin is a potent antifibrotic agent that may have therapeutic potential for patients with epidural fibrosis.
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spelling pubmed-50456102016-10-12 The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats Dai, Jihang Li, Xiaolei Yan, Lianqi Chen, Hui He, Jun Wang, Shuguang Wang, Jingcheng Sun, Yu J Orthop Surg Res Research Article BACKGROUND: Epidural fibrosis often causes serious complications in patients after lumbar laminectomy and discectomy and is associated with the proliferation of fibroblasts. Suramin is known to have an obvious inhibitory effect on the coactions of many growth factors and their receptors, but little was previously known about the effect of suramin on fibroblast proliferation and the progress of epidural fibrosis. METHODS: We illustrated the effect of suramin on cultured fibroblasts of rats with different concentrations (0, 200, 400, 600 mg/l). The proliferation of suramin-treated fibroblasts was evaluated by CCK-8 and western blot analysis. Additionally, in a rat model of laminectomy, different concentrations of suramin (100, 200, and 300 mg/ml) and saline were applied to the laminectomy sites locally. The effect of suramin on preventing epidural fibrosis was detected by the Rydell classification, hydroxyproline content, histological analysis, and collagen density analyses. RESULTS: The results of CCK-8 shown that suramin could significantly inhibit fibroblasts proliferation in a dose-dependent manner. The western blotting shown that the expression levels of the cell proliferation markers such as cyclin D1, cyclin E, and PCNA were down-regulated. Moreover, in a rat model, we found that suramin could reduce epidural fibrosis as well as inhibit fibroblast proliferation, and 300 mg/ml suramin had better effect. CONCLUSIONS: Topical application of suramin could reduce epidural fibrosis after laminectomy, and the application of suramin could inhibit the proliferation of fibroblasts in rats. This study indicates that suramin is a potent antifibrotic agent that may have therapeutic potential for patients with epidural fibrosis. BioMed Central 2016-10-01 /pmc/articles/PMC5045610/ /pubmed/27716371 http://dx.doi.org/10.1186/s13018-016-0443-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Dai, Jihang
Li, Xiaolei
Yan, Lianqi
Chen, Hui
He, Jun
Wang, Shuguang
Wang, Jingcheng
Sun, Yu
The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats
title The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats
title_full The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats
title_fullStr The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats
title_full_unstemmed The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats
title_short The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats
title_sort effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045610/
https://www.ncbi.nlm.nih.gov/pubmed/27716371
http://dx.doi.org/10.1186/s13018-016-0443-5
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