XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 tr...

Descripción completa

Detalles Bibliográficos
Autores principales: Thomas, Nicholas C., Schwessinger, Benjamin, Liu, Furong, Chen, Huamin, Wei, Tong, Nguyen, Yen P., Shaker, Isaac W.F., Ronald, Pamela C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Agricultural Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045893/
https://www.ncbi.nlm.nih.gov/pubmed/27703843
http://dx.doi.org/10.7717/peerj.2446
Descripción
Sumario:The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.

Ejemplares similares