XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 tr...

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Autores principales: Thomas, Nicholas C., Schwessinger, Benjamin, Liu, Furong, Chen, Huamin, Wei, Tong, Nguyen, Yen P., Shaker, Isaac W.F., Ronald, Pamela C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Agricultural Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045893/
https://www.ncbi.nlm.nih.gov/pubmed/27703843
http://dx.doi.org/10.7717/peerj.2446
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author Thomas, Nicholas C.
Schwessinger, Benjamin
Liu, Furong
Chen, Huamin
Wei, Tong
Nguyen, Yen P.
Shaker, Isaac W.F.
Ronald, Pamela C.
author_facet Thomas, Nicholas C.
Schwessinger, Benjamin
Liu, Furong
Chen, Huamin
Wei, Tong
Nguyen, Yen P.
Shaker, Isaac W.F.
Ronald, Pamela C.
author_sort Thomas, Nicholas C.
collection PubMed
description The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.
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spelling pubmed-50458932016-10-04 XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves Thomas, Nicholas C. Schwessinger, Benjamin Liu, Furong Chen, Huamin Wei, Tong Nguyen, Yen P. Shaker, Isaac W.F. Ronald, Pamela C. PeerJ Agricultural Science The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions. PeerJ Inc. 2016-09-28 /pmc/articles/PMC5045893/ /pubmed/27703843 http://dx.doi.org/10.7717/peerj.2446 Text en ©2016 Thomas et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Thomas, Nicholas C.
Schwessinger, Benjamin
Liu, Furong
Chen, Huamin
Wei, Tong
Nguyen, Yen P.
Shaker, Isaac W.F.
Ronald, Pamela C.
XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves
title XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves
title_full XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves
title_fullStr XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves
title_full_unstemmed XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves
title_short XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves
title_sort xa21-specific induction of stress-related genes following xanthomonas infection of detached rice leaves
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5045893/
https://www.ncbi.nlm.nih.gov/pubmed/27703843
http://dx.doi.org/10.7717/peerj.2446
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