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Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production

Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release...

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Autores principales: Berłowska, Joanna, Pielech-Przybylska, Katarzyna, Balcerek, Maria, Dziekońska-Kubczak, Urszula, Patelski, Piotr, Dziugan, Piotr, Kręgiel, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5046097/
https://www.ncbi.nlm.nih.gov/pubmed/27722169
http://dx.doi.org/10.1155/2016/3154929
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author Berłowska, Joanna
Pielech-Przybylska, Katarzyna
Balcerek, Maria
Dziekońska-Kubczak, Urszula
Patelski, Piotr
Dziugan, Piotr
Kręgiel, Dorota
author_facet Berłowska, Joanna
Pielech-Przybylska, Katarzyna
Balcerek, Maria
Dziekońska-Kubczak, Urszula
Patelski, Piotr
Dziugan, Piotr
Kręgiel, Dorota
author_sort Berłowska, Joanna
collection PubMed
description Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015–0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red (S. cerevisiae) (1 g/L) and Pichia stipitis (0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of sugar beet pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching 26.9 ± 1.2 g/L and 86.5 ± 2.1% fermentation efficiency relative to the theoretical yield.
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spelling pubmed-50460972016-10-09 Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production Berłowska, Joanna Pielech-Przybylska, Katarzyna Balcerek, Maria Dziekońska-Kubczak, Urszula Patelski, Piotr Dziugan, Piotr Kręgiel, Dorota Biomed Res Int Research Article Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015–0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red (S. cerevisiae) (1 g/L) and Pichia stipitis (0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of sugar beet pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching 26.9 ± 1.2 g/L and 86.5 ± 2.1% fermentation efficiency relative to the theoretical yield. Hindawi Publishing Corporation 2016 2016-09-19 /pmc/articles/PMC5046097/ /pubmed/27722169 http://dx.doi.org/10.1155/2016/3154929 Text en Copyright © 2016 Joanna Berłowska et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Berłowska, Joanna
Pielech-Przybylska, Katarzyna
Balcerek, Maria
Dziekońska-Kubczak, Urszula
Patelski, Piotr
Dziugan, Piotr
Kręgiel, Dorota
Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production
title Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production
title_full Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production
title_fullStr Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production
title_full_unstemmed Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production
title_short Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production
title_sort simultaneous saccharification and fermentation of sugar beet pulp for efficient bioethanol production
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5046097/
https://www.ncbi.nlm.nih.gov/pubmed/27722169
http://dx.doi.org/10.1155/2016/3154929
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