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Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage

Newcastle disease (ND), caused by virulent strains of Newcastle disease virus (NDV), is a highly contagious disease of birds that is responsible for heavy economic losses for the poultry industry worldwide. However, little is known about host-virus interactions in waterfowl, goose. In this study, we...

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Autores principales: Xu, Qianqian, Chen, Yuqiu, Zhao, Wenjun, Zhang, Tingting, Liu, Chenggang, Qi, Tianming, Han, Zongxi, Shao, Yuhao, Ma, Deying, Liu, Shengwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047883/
https://www.ncbi.nlm.nih.gov/pubmed/27757109
http://dx.doi.org/10.3389/fmicb.2016.01587
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author Xu, Qianqian
Chen, Yuqiu
Zhao, Wenjun
Zhang, Tingting
Liu, Chenggang
Qi, Tianming
Han, Zongxi
Shao, Yuhao
Ma, Deying
Liu, Shengwang
author_facet Xu, Qianqian
Chen, Yuqiu
Zhao, Wenjun
Zhang, Tingting
Liu, Chenggang
Qi, Tianming
Han, Zongxi
Shao, Yuhao
Ma, Deying
Liu, Shengwang
author_sort Xu, Qianqian
collection PubMed
description Newcastle disease (ND), caused by virulent strains of Newcastle disease virus (NDV), is a highly contagious disease of birds that is responsible for heavy economic losses for the poultry industry worldwide. However, little is known about host-virus interactions in waterfowl, goose. In this study, we aim to characterize the host immune response in goose, based on the previous reports on the host response to NDV in chickens. Here, we evaluated viral replication and mRNA expression of 27 immune-related genes in 10 tissues of geese challenged with a genotype VIId NDV strain of goose origin (go/CH/LHLJ/1/06). The virus showed early replication, especially in digestive and immune tissues. The expression profiles showed up-regulation of Toll-like receptor (TLR)1–3, 5, 7, and 15, avian β-defensin (AvBD) 5–7, 10, 12, and 16, cytokines [interleukin (IL)-8, IL-18, IL-1β, and interferon-γ], inducible NO synthase (iNOS), and MHC class I in some tissues of geese in response to NDV. In contrast, NDV infection suppressed expression of AvBD1 in cecal tonsil of geese. Moreover, we observed a highly positive correlation between viral replication and host mRNA expressions of TLR1-5 and 7, AvBD4-6, 10, and 12, all the cytokines measured, MHC class I, FAS ligand, and iNOS, mainly at 72 h post-infection. Taken together, these results demonstrated that NDV infection induces strong innate immune responses and intense inflammatory responses at early stage in goose which may associate with the viral pathogenesis.
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spelling pubmed-50478832016-10-18 Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage Xu, Qianqian Chen, Yuqiu Zhao, Wenjun Zhang, Tingting Liu, Chenggang Qi, Tianming Han, Zongxi Shao, Yuhao Ma, Deying Liu, Shengwang Front Microbiol Microbiology Newcastle disease (ND), caused by virulent strains of Newcastle disease virus (NDV), is a highly contagious disease of birds that is responsible for heavy economic losses for the poultry industry worldwide. However, little is known about host-virus interactions in waterfowl, goose. In this study, we aim to characterize the host immune response in goose, based on the previous reports on the host response to NDV in chickens. Here, we evaluated viral replication and mRNA expression of 27 immune-related genes in 10 tissues of geese challenged with a genotype VIId NDV strain of goose origin (go/CH/LHLJ/1/06). The virus showed early replication, especially in digestive and immune tissues. The expression profiles showed up-regulation of Toll-like receptor (TLR)1–3, 5, 7, and 15, avian β-defensin (AvBD) 5–7, 10, 12, and 16, cytokines [interleukin (IL)-8, IL-18, IL-1β, and interferon-γ], inducible NO synthase (iNOS), and MHC class I in some tissues of geese in response to NDV. In contrast, NDV infection suppressed expression of AvBD1 in cecal tonsil of geese. Moreover, we observed a highly positive correlation between viral replication and host mRNA expressions of TLR1-5 and 7, AvBD4-6, 10, and 12, all the cytokines measured, MHC class I, FAS ligand, and iNOS, mainly at 72 h post-infection. Taken together, these results demonstrated that NDV infection induces strong innate immune responses and intense inflammatory responses at early stage in goose which may associate with the viral pathogenesis. Frontiers Media S.A. 2016-10-04 /pmc/articles/PMC5047883/ /pubmed/27757109 http://dx.doi.org/10.3389/fmicb.2016.01587 Text en Copyright © 2016 Xu, Chen, Zhao, Zhang, Liu, Qi, Han, Shao, Ma and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xu, Qianqian
Chen, Yuqiu
Zhao, Wenjun
Zhang, Tingting
Liu, Chenggang
Qi, Tianming
Han, Zongxi
Shao, Yuhao
Ma, Deying
Liu, Shengwang
Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage
title Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage
title_full Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage
title_fullStr Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage
title_full_unstemmed Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage
title_short Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage
title_sort infection of goose with genotype viid newcastle disease virus of goose origin elicits strong immune responses at early stage
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047883/
https://www.ncbi.nlm.nih.gov/pubmed/27757109
http://dx.doi.org/10.3389/fmicb.2016.01587
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