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Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent

Acinetobacter baumannii is a gram-negative, non-motile bacterium that, due to its multidrug resistance, has become a major nosocomial pathogen. The increasing number of multidrug resistant (MDR) strains has renewed interest in phage therapy. The aim of our study was to assess the effectiveness of ph...

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Autores principales: Kusradze, Ia, Karumidze, Natia, Rigvava, Sophio, Dvalidze, Teona, Katsitadze, Malkhaz, Amiranashvili, Irakli, Goderdzishvili, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047890/
https://www.ncbi.nlm.nih.gov/pubmed/27757110
http://dx.doi.org/10.3389/fmicb.2016.01590
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author Kusradze, Ia
Karumidze, Natia
Rigvava, Sophio
Dvalidze, Teona
Katsitadze, Malkhaz
Amiranashvili, Irakli
Goderdzishvili, Marina
author_facet Kusradze, Ia
Karumidze, Natia
Rigvava, Sophio
Dvalidze, Teona
Katsitadze, Malkhaz
Amiranashvili, Irakli
Goderdzishvili, Marina
author_sort Kusradze, Ia
collection PubMed
description Acinetobacter baumannii is a gram-negative, non-motile bacterium that, due to its multidrug resistance, has become a major nosocomial pathogen. The increasing number of multidrug resistant (MDR) strains has renewed interest in phage therapy. The aim of our study was to assess the effectiveness of phage administration in Acinetobacter baumannii wound infections in an animal model to demonstrate phage therapy as non-toxic, safe and alternative antibacterial remedy. Using classical methods for the study of bacteriophage properties, we characterized phage vB-GEC_Ab-M-G7 as a dsDNA myovirus with a 90 kb genome size. Important characteristics of vB-GEC_Ab-M-G7include a short latent period and large burst size, wide host range, resistance to chloroform and thermal and pH stability. In a rat wound model, phage application effectively decreased the number of bacteria isolated from the wounds of successfully treated animals. This study highlights the effectiveness of the phage therapy and provides further insight into treating infections caused by MDR strains using phage administration.
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spelling pubmed-50478902016-10-18 Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent Kusradze, Ia Karumidze, Natia Rigvava, Sophio Dvalidze, Teona Katsitadze, Malkhaz Amiranashvili, Irakli Goderdzishvili, Marina Front Microbiol Microbiology Acinetobacter baumannii is a gram-negative, non-motile bacterium that, due to its multidrug resistance, has become a major nosocomial pathogen. The increasing number of multidrug resistant (MDR) strains has renewed interest in phage therapy. The aim of our study was to assess the effectiveness of phage administration in Acinetobacter baumannii wound infections in an animal model to demonstrate phage therapy as non-toxic, safe and alternative antibacterial remedy. Using classical methods for the study of bacteriophage properties, we characterized phage vB-GEC_Ab-M-G7 as a dsDNA myovirus with a 90 kb genome size. Important characteristics of vB-GEC_Ab-M-G7include a short latent period and large burst size, wide host range, resistance to chloroform and thermal and pH stability. In a rat wound model, phage application effectively decreased the number of bacteria isolated from the wounds of successfully treated animals. This study highlights the effectiveness of the phage therapy and provides further insight into treating infections caused by MDR strains using phage administration. Frontiers Media S.A. 2016-10-04 /pmc/articles/PMC5047890/ /pubmed/27757110 http://dx.doi.org/10.3389/fmicb.2016.01590 Text en Copyright © 2016 Kusradze, Karumidze, Rigvava, Dvalidze, Katsitadze, Amiranashvili and Goderdzishvili. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kusradze, Ia
Karumidze, Natia
Rigvava, Sophio
Dvalidze, Teona
Katsitadze, Malkhaz
Amiranashvili, Irakli
Goderdzishvili, Marina
Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent
title Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent
title_full Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent
title_fullStr Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent
title_full_unstemmed Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent
title_short Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent
title_sort characterization and testing the efficiency of acinetobacter baumannii phage vb-gec_ab-m-g7 as an antibacterial agent
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047890/
https://www.ncbi.nlm.nih.gov/pubmed/27757110
http://dx.doi.org/10.3389/fmicb.2016.01590
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