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Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines

In the last few years, marine species have been investigated for the presence of natural products with anticancer activity. Using reversed phase chromatography, low molecular weight proteins were fractionated from the sea anemone Anemonia viridis. Four different fractions were evaluated for their cy...

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Autores principales: Bulati, Matteo, Longo, Alessandra, Masullo, Tiziana, Vlah, Sara, Bennici, Carmelo, Bonura, Angela, Salamone, Monica, Tagliavia, Marcello, Nicosia, Aldo, Mazzola, Salvatore, Colombo, Paolo, Cuttitta, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048049/
https://www.ncbi.nlm.nih.gov/pubmed/27725939
http://dx.doi.org/10.1155/2016/3849897
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author Bulati, Matteo
Longo, Alessandra
Masullo, Tiziana
Vlah, Sara
Bennici, Carmelo
Bonura, Angela
Salamone, Monica
Tagliavia, Marcello
Nicosia, Aldo
Mazzola, Salvatore
Colombo, Paolo
Cuttitta, Angela
author_facet Bulati, Matteo
Longo, Alessandra
Masullo, Tiziana
Vlah, Sara
Bennici, Carmelo
Bonura, Angela
Salamone, Monica
Tagliavia, Marcello
Nicosia, Aldo
Mazzola, Salvatore
Colombo, Paolo
Cuttitta, Angela
author_sort Bulati, Matteo
collection PubMed
description In the last few years, marine species have been investigated for the presence of natural products with anticancer activity. Using reversed phase chromatography, low molecular weight proteins were fractionated from the sea anemone Anemonia viridis. Four different fractions were evaluated for their cytotoxic activity by means of erythrocyte haemolysis test, MTS, and LDH assays. Finally, the antiproliferative activities of three of these fractions were studied on PC3, PLC/PRF/5, and A375 human cancer cell lines. Our analysis revealed that the four fractions showed different protein contents and diverse patterns of activity towards human PBMC and cancer cell lines. Interestingly, fractions III and IV exerted cytotoxic effects on human cells. Conversely, fractions I and II displayed very low toxic effects associated with antiproliferative activities on cancer cell lines.
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spelling pubmed-50480492016-10-10 Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines Bulati, Matteo Longo, Alessandra Masullo, Tiziana Vlah, Sara Bennici, Carmelo Bonura, Angela Salamone, Monica Tagliavia, Marcello Nicosia, Aldo Mazzola, Salvatore Colombo, Paolo Cuttitta, Angela Biomed Res Int Research Article In the last few years, marine species have been investigated for the presence of natural products with anticancer activity. Using reversed phase chromatography, low molecular weight proteins were fractionated from the sea anemone Anemonia viridis. Four different fractions were evaluated for their cytotoxic activity by means of erythrocyte haemolysis test, MTS, and LDH assays. Finally, the antiproliferative activities of three of these fractions were studied on PC3, PLC/PRF/5, and A375 human cancer cell lines. Our analysis revealed that the four fractions showed different protein contents and diverse patterns of activity towards human PBMC and cancer cell lines. Interestingly, fractions III and IV exerted cytotoxic effects on human cells. Conversely, fractions I and II displayed very low toxic effects associated with antiproliferative activities on cancer cell lines. Hindawi Publishing Corporation 2016 2016-09-20 /pmc/articles/PMC5048049/ /pubmed/27725939 http://dx.doi.org/10.1155/2016/3849897 Text en Copyright © 2016 Matteo Bulati et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bulati, Matteo
Longo, Alessandra
Masullo, Tiziana
Vlah, Sara
Bennici, Carmelo
Bonura, Angela
Salamone, Monica
Tagliavia, Marcello
Nicosia, Aldo
Mazzola, Salvatore
Colombo, Paolo
Cuttitta, Angela
Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines
title Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines
title_full Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines
title_fullStr Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines
title_full_unstemmed Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines
title_short Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines
title_sort partially purified extracts of sea anemone anemonia viridis affect the growth and viability of selected tumour cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048049/
https://www.ncbi.nlm.nih.gov/pubmed/27725939
http://dx.doi.org/10.1155/2016/3849897
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