Cargando…

Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices

The recent success of olfactory ensheathing cell (OEC) assisted regeneration of injured spinal cord has seen a rising interest in the use of these cells in tissue-engineered systems. Previously shown to support neural cell growth through glial scar tissue, OECs have the potential to assist neural ne...

Descripción completa

Detalles Bibliográficos
Autores principales: Hassarati, Rachelle T., Foster, L. John R., Green, Rylie A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048075/
https://www.ncbi.nlm.nih.gov/pubmed/27757072
http://dx.doi.org/10.3389/fnins.2016.00432
_version_ 1782457532579577856
author Hassarati, Rachelle T.
Foster, L. John R.
Green, Rylie A.
author_facet Hassarati, Rachelle T.
Foster, L. John R.
Green, Rylie A.
author_sort Hassarati, Rachelle T.
collection PubMed
description The recent success of olfactory ensheathing cell (OEC) assisted regeneration of injured spinal cord has seen a rising interest in the use of these cells in tissue-engineered systems. Previously shown to support neural cell growth through glial scar tissue, OECs have the potential to assist neural network formation in living electrode systems to produce superior neuroprosthetic electrode surfaces. The following study sought to understand the influence of biphasic electrical stimulation (ES), inherent to bionic devices, on cell survival and function, with respect to conventional metallic and developmental conductive hydrogel (CH) coated electrodes. The CH utilized in this study was a biosynthetic hydrogel consisting of methacrylated poly(vinyl-alcohol) (PVA), heparin and gelatin through which poly(3,4-ethylenedioxythiophene) (PEDOT) was electropolymerised. OECs cultured on Pt and CH surfaces were subjected to biphasic ES. Image-based cytometry yielded little significant difference between the viability and cell cycle of OECs cultured on the stimulated and passive samples. The significantly lower voltages measured across the CH electrodes (147 ± 3 mV) compared to the Pt (317 ± 5 mV), had shown to influence a higher percentage of viable cells on CH (91–93%) compared to Pt (78–81%). To determine the functionality of these cells following electrical stimulation, OECs co-cultured with PC12 cells were found to support neural cell differentiation (an indirect measure of neurotrophic factor production) following ES.
format Online
Article
Text
id pubmed-5048075
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-50480752016-10-18 Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices Hassarati, Rachelle T. Foster, L. John R. Green, Rylie A. Front Neurosci Neuroscience The recent success of olfactory ensheathing cell (OEC) assisted regeneration of injured spinal cord has seen a rising interest in the use of these cells in tissue-engineered systems. Previously shown to support neural cell growth through glial scar tissue, OECs have the potential to assist neural network formation in living electrode systems to produce superior neuroprosthetic electrode surfaces. The following study sought to understand the influence of biphasic electrical stimulation (ES), inherent to bionic devices, on cell survival and function, with respect to conventional metallic and developmental conductive hydrogel (CH) coated electrodes. The CH utilized in this study was a biosynthetic hydrogel consisting of methacrylated poly(vinyl-alcohol) (PVA), heparin and gelatin through which poly(3,4-ethylenedioxythiophene) (PEDOT) was electropolymerised. OECs cultured on Pt and CH surfaces were subjected to biphasic ES. Image-based cytometry yielded little significant difference between the viability and cell cycle of OECs cultured on the stimulated and passive samples. The significantly lower voltages measured across the CH electrodes (147 ± 3 mV) compared to the Pt (317 ± 5 mV), had shown to influence a higher percentage of viable cells on CH (91–93%) compared to Pt (78–81%). To determine the functionality of these cells following electrical stimulation, OECs co-cultured with PC12 cells were found to support neural cell differentiation (an indirect measure of neurotrophic factor production) following ES. Frontiers Media S.A. 2016-10-04 /pmc/articles/PMC5048075/ /pubmed/27757072 http://dx.doi.org/10.3389/fnins.2016.00432 Text en Copyright © 2016 Hassarati, Foster and Green. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Hassarati, Rachelle T.
Foster, L. John R.
Green, Rylie A.
Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices
title Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices
title_full Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices
title_fullStr Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices
title_full_unstemmed Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices
title_short Influence of Biphasic Stimulation on Olfactory Ensheathing Cells for Neuroprosthetic Devices
title_sort influence of biphasic stimulation on olfactory ensheathing cells for neuroprosthetic devices
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048075/
https://www.ncbi.nlm.nih.gov/pubmed/27757072
http://dx.doi.org/10.3389/fnins.2016.00432
work_keys_str_mv AT hassaratirachellet influenceofbiphasicstimulationonolfactoryensheathingcellsforneuroprostheticdevices
AT fosterljohnr influenceofbiphasicstimulationonolfactoryensheathingcellsforneuroprostheticdevices
AT greenryliea influenceofbiphasicstimulationonolfactoryensheathingcellsforneuroprostheticdevices