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Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat

OBJECTIVE(S): The aim of this study was to estimate effects of hyperbaric (HB) treatment by determination of CD15s and CD11b leukocyte proinflammatory markers expression. In addition, this study describes changes in CD77 and CD34 expression on rat endothelial cells in renal, pulmonary and cardiac ti...

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Autores principales: Đevenica, Danka, Markotić, Anita, Režić-Mužinić, Nikolina, Jelaska, Igor, Zemunik, Tatijana, Delić, Hrvoje, Čulić, Vedrana Čikeš
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048116/
https://www.ncbi.nlm.nih.gov/pubmed/27746862
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author Đevenica, Danka
Markotić, Anita
Režić-Mužinić, Nikolina
Jelaska, Igor
Zemunik, Tatijana
Delić, Hrvoje
Čulić, Vedrana Čikeš
author_facet Đevenica, Danka
Markotić, Anita
Režić-Mužinić, Nikolina
Jelaska, Igor
Zemunik, Tatijana
Delić, Hrvoje
Čulić, Vedrana Čikeš
author_sort Đevenica, Danka
collection PubMed
description OBJECTIVE(S): The aim of this study was to estimate effects of hyperbaric (HB) treatment by determination of CD15s and CD11b leukocyte proinflammatory markers expression. In addition, this study describes changes in CD77 and CD34 expression on rat endothelial cells in renal, pulmonary and cardiac tissue following exposure to hyperbaric pressure. MATERIALS AND METHODS: Expression of CD11b and CD15s on leukocytes, as well as CD77 and CD34 expression on endothelial cells in cell suspensions of renal, pulmonary and cardiac tissue in rats after hyperbaric treatment and in control rats were determined by flow cytometry. RESULTS: Hyperbaric treatment significantly increased percentage of leukocytes expressing CD15s+CD11b- (from 1.71±1.11 to 23.42±2.85, P<0.05). Hyperbaric treatment significantly decreased sum percentage of CD77+CD34- and CD77+CD34+ renal cells (from 16.35±5.5 to 4.48 ±1.28, P<0.05). Hyperbaric treatment significantly increased percentage of CD34+ pulmonary cells (from 3.27±2.01 to 11.92±6.22, P<0.05). Our study is the first reporting the hyperbaric environment influence on CD34+ heart cells in rats. CONCLUSION: The current findings of increased percentage of leukocytes expressing endothelial selectin ligand CD15s after hyperbaric treatment, point its role in endothelial damage prevention. We found out a significantly increase in percentage of CD34+ cardiac cells as well as CD34+ pulmonary cells in rats after HB treatment which could be a part of repair mechanism of injured endothelium caused by hyperoxia.
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spelling pubmed-50481162016-10-14 Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat Đevenica, Danka Markotić, Anita Režić-Mužinić, Nikolina Jelaska, Igor Zemunik, Tatijana Delić, Hrvoje Čulić, Vedrana Čikeš Iran J Basic Med Sci Original Article OBJECTIVE(S): The aim of this study was to estimate effects of hyperbaric (HB) treatment by determination of CD15s and CD11b leukocyte proinflammatory markers expression. In addition, this study describes changes in CD77 and CD34 expression on rat endothelial cells in renal, pulmonary and cardiac tissue following exposure to hyperbaric pressure. MATERIALS AND METHODS: Expression of CD11b and CD15s on leukocytes, as well as CD77 and CD34 expression on endothelial cells in cell suspensions of renal, pulmonary and cardiac tissue in rats after hyperbaric treatment and in control rats were determined by flow cytometry. RESULTS: Hyperbaric treatment significantly increased percentage of leukocytes expressing CD15s+CD11b- (from 1.71±1.11 to 23.42±2.85, P<0.05). Hyperbaric treatment significantly decreased sum percentage of CD77+CD34- and CD77+CD34+ renal cells (from 16.35±5.5 to 4.48 ±1.28, P<0.05). Hyperbaric treatment significantly increased percentage of CD34+ pulmonary cells (from 3.27±2.01 to 11.92±6.22, P<0.05). Our study is the first reporting the hyperbaric environment influence on CD34+ heart cells in rats. CONCLUSION: The current findings of increased percentage of leukocytes expressing endothelial selectin ligand CD15s after hyperbaric treatment, point its role in endothelial damage prevention. We found out a significantly increase in percentage of CD34+ cardiac cells as well as CD34+ pulmonary cells in rats after HB treatment which could be a part of repair mechanism of injured endothelium caused by hyperoxia. Mashhad University of Medical Sciences 2016-08 /pmc/articles/PMC5048116/ /pubmed/27746862 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Đevenica, Danka
Markotić, Anita
Režić-Mužinić, Nikolina
Jelaska, Igor
Zemunik, Tatijana
Delić, Hrvoje
Čulić, Vedrana Čikeš
Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat
title Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat
title_full Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat
title_fullStr Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat
title_full_unstemmed Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat
title_short Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat
title_sort hyperbaric environment up-regulates cd15s expression on leukocytes, down-regulates cd77 expression on renal cells and up-regulates cd34 expression on pulmonary and cardiac cells in rat
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048116/
https://www.ncbi.nlm.nih.gov/pubmed/27746862
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