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Characterization of a very rare case of living ewe-buck hybrid using classical and molecular cytogenetics
The natural occurrence of live hybrid offsprings between sheep and goats has been documented in literature, however all the studies have reported the mating of goats with rams, whereas the reciprocal cross was never documented. This study reports on a very rare case of interspecies hybridization occ...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048133/ https://www.ncbi.nlm.nih.gov/pubmed/27698378 http://dx.doi.org/10.1038/srep34781 |
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author | Pauciullo, Alfredo Knorr, Christoph Perucatti, Angela Iannuzzi, Alessandra Iannuzzi, Leopoldo Erhardt, Georg |
author_facet | Pauciullo, Alfredo Knorr, Christoph Perucatti, Angela Iannuzzi, Alessandra Iannuzzi, Leopoldo Erhardt, Georg |
author_sort | Pauciullo, Alfredo |
collection | PubMed |
description | The natural occurrence of live hybrid offsprings between sheep and goats has been documented in literature, however all the studies have reported the mating of goats with rams, whereas the reciprocal cross was never documented. This study reports on a very rare case of interspecies hybridization occurred between a ewe (2n = 54, XX) and a buck (2n = 60, XY). The hybrid, born in a German flock under natural conditions, is characterised by an intermediate karyotype (2n = 57, XX). The CBA-banding has shown 3 metacentric and 54 acrocentric chromosomes, whereas the GTG- and RBA-banding have revealed that the autosomes involved in the hybrid combination were CHI1, 3; CHI2, 8 and CHI5, 11 corresponding to the metacentric chromosomes OAR1, OAR2 and OAR3. A tri-colour FISH using chromosome paintings and BAC probes has validated this arrangement. A further FISH analysis has been carried out to analyse the telomeres, which showed a normal structure. Nucleolus organiser-bearing chromosomes were identified as pairs OAR1p(CHI3), OAR2q(CHI2), OAR3q(CHI5), OAR4(CHI4) and OAR25(CHI28), and nuclear associations were found. Sex chromosomes were correctly arranged. The odd number of the karyotype might be responsible for a reduced fertility as consequence of the incorrect chromosomal pairing and/or segregation during the meiosis. |
format | Online Article Text |
id | pubmed-5048133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50481332016-10-11 Characterization of a very rare case of living ewe-buck hybrid using classical and molecular cytogenetics Pauciullo, Alfredo Knorr, Christoph Perucatti, Angela Iannuzzi, Alessandra Iannuzzi, Leopoldo Erhardt, Georg Sci Rep Article The natural occurrence of live hybrid offsprings between sheep and goats has been documented in literature, however all the studies have reported the mating of goats with rams, whereas the reciprocal cross was never documented. This study reports on a very rare case of interspecies hybridization occurred between a ewe (2n = 54, XX) and a buck (2n = 60, XY). The hybrid, born in a German flock under natural conditions, is characterised by an intermediate karyotype (2n = 57, XX). The CBA-banding has shown 3 metacentric and 54 acrocentric chromosomes, whereas the GTG- and RBA-banding have revealed that the autosomes involved in the hybrid combination were CHI1, 3; CHI2, 8 and CHI5, 11 corresponding to the metacentric chromosomes OAR1, OAR2 and OAR3. A tri-colour FISH using chromosome paintings and BAC probes has validated this arrangement. A further FISH analysis has been carried out to analyse the telomeres, which showed a normal structure. Nucleolus organiser-bearing chromosomes were identified as pairs OAR1p(CHI3), OAR2q(CHI2), OAR3q(CHI5), OAR4(CHI4) and OAR25(CHI28), and nuclear associations were found. Sex chromosomes were correctly arranged. The odd number of the karyotype might be responsible for a reduced fertility as consequence of the incorrect chromosomal pairing and/or segregation during the meiosis. Nature Publishing Group 2016-10-04 /pmc/articles/PMC5048133/ /pubmed/27698378 http://dx.doi.org/10.1038/srep34781 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Pauciullo, Alfredo Knorr, Christoph Perucatti, Angela Iannuzzi, Alessandra Iannuzzi, Leopoldo Erhardt, Georg Characterization of a very rare case of living ewe-buck hybrid using classical and molecular cytogenetics |
title | Characterization of a very rare case of living ewe-buck hybrid using classical and molecular cytogenetics |
title_full | Characterization of a very rare case of living ewe-buck hybrid using classical and molecular cytogenetics |
title_fullStr | Characterization of a very rare case of living ewe-buck hybrid using classical and molecular cytogenetics |
title_full_unstemmed | Characterization of a very rare case of living ewe-buck hybrid using classical and molecular cytogenetics |
title_short | Characterization of a very rare case of living ewe-buck hybrid using classical and molecular cytogenetics |
title_sort | characterization of a very rare case of living ewe-buck hybrid using classical and molecular cytogenetics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048133/ https://www.ncbi.nlm.nih.gov/pubmed/27698378 http://dx.doi.org/10.1038/srep34781 |
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