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Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice

The endotoxin lipopolysaccharide (LPS) promotes sepsis, but bacterial peptides also promote inflammation leading to sepsis. We found, intraperitoneal administration of live or heat inactivated E. coli JE5505 lacking the abundant outer membrane protein, Braun lipoprotein (BLP), was less toxic than E....

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Autores principales: Lakshmikanth, Chikkamenahalli Lakshminarayana, Jacob, Shancy Petsel, Kudva, Avinash Kundadka, Latchoumycandane, Calivarathan, Yashaswini, Puttaraju Srikanta Murthy, Sumanth, Mosale Seetharam, Goncalves-de-Albuquerque, Cassiano F., Silva, Adriana R., Singh, Sridevi Annapurna, Castro-Faria-Neto, Hugo C., Prabhu, Sandeep Kumble, McIntyre, Thomas M., Marathe, Gopal Kedihithlu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048175/
https://www.ncbi.nlm.nih.gov/pubmed/27698491
http://dx.doi.org/10.1038/srep34666
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author Lakshmikanth, Chikkamenahalli Lakshminarayana
Jacob, Shancy Petsel
Kudva, Avinash Kundadka
Latchoumycandane, Calivarathan
Yashaswini, Puttaraju Srikanta Murthy
Sumanth, Mosale Seetharam
Goncalves-de-Albuquerque, Cassiano F.
Silva, Adriana R.
Singh, Sridevi Annapurna
Castro-Faria-Neto, Hugo C.
Prabhu, Sandeep Kumble
McIntyre, Thomas M.
Marathe, Gopal Kedihithlu
author_facet Lakshmikanth, Chikkamenahalli Lakshminarayana
Jacob, Shancy Petsel
Kudva, Avinash Kundadka
Latchoumycandane, Calivarathan
Yashaswini, Puttaraju Srikanta Murthy
Sumanth, Mosale Seetharam
Goncalves-de-Albuquerque, Cassiano F.
Silva, Adriana R.
Singh, Sridevi Annapurna
Castro-Faria-Neto, Hugo C.
Prabhu, Sandeep Kumble
McIntyre, Thomas M.
Marathe, Gopal Kedihithlu
author_sort Lakshmikanth, Chikkamenahalli Lakshminarayana
collection PubMed
description The endotoxin lipopolysaccharide (LPS) promotes sepsis, but bacterial peptides also promote inflammation leading to sepsis. We found, intraperitoneal administration of live or heat inactivated E. coli JE5505 lacking the abundant outer membrane protein, Braun lipoprotein (BLP), was less toxic than E. coli DH5α possessing BLP in Swiss albino mice. Injection of BLP free of LPS purified from E. coli DH5α induced massive infiltration of leukocytes in lungs and liver. BLP activated human polymorphonuclear cells (PMNs) ex vivo to adhere to denatured collagen in serum and polymyxin B independent fashion, a property distinct from LPS. Both LPS and BLP stimulated the synthesis of platelet activating factor (PAF), a potent lipid mediator, in human PMNs. In mouse macrophage cell line, RAW264.7, while both BLP and LPS similarly upregulated TNF-α and IL-1β mRNA; BLP was more potent in inducing cyclooxygenase-2 (COX-2) mRNA and protein expression. Peritoneal macrophages from TLR2(−/−) mice significantly reduced the production of TNF-α in response to BLP in contrast to macrophages from wild type mice. We conclude, BLP acting through TLR2, is a potent inducer of inflammation with a response profile both common and distinct from LPS. Hence, BLP mediated pathway may also be considered as an effective target against sepsis.
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spelling pubmed-50481752016-10-11 Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice Lakshmikanth, Chikkamenahalli Lakshminarayana Jacob, Shancy Petsel Kudva, Avinash Kundadka Latchoumycandane, Calivarathan Yashaswini, Puttaraju Srikanta Murthy Sumanth, Mosale Seetharam Goncalves-de-Albuquerque, Cassiano F. Silva, Adriana R. Singh, Sridevi Annapurna Castro-Faria-Neto, Hugo C. Prabhu, Sandeep Kumble McIntyre, Thomas M. Marathe, Gopal Kedihithlu Sci Rep Article The endotoxin lipopolysaccharide (LPS) promotes sepsis, but bacterial peptides also promote inflammation leading to sepsis. We found, intraperitoneal administration of live or heat inactivated E. coli JE5505 lacking the abundant outer membrane protein, Braun lipoprotein (BLP), was less toxic than E. coli DH5α possessing BLP in Swiss albino mice. Injection of BLP free of LPS purified from E. coli DH5α induced massive infiltration of leukocytes in lungs and liver. BLP activated human polymorphonuclear cells (PMNs) ex vivo to adhere to denatured collagen in serum and polymyxin B independent fashion, a property distinct from LPS. Both LPS and BLP stimulated the synthesis of platelet activating factor (PAF), a potent lipid mediator, in human PMNs. In mouse macrophage cell line, RAW264.7, while both BLP and LPS similarly upregulated TNF-α and IL-1β mRNA; BLP was more potent in inducing cyclooxygenase-2 (COX-2) mRNA and protein expression. Peritoneal macrophages from TLR2(−/−) mice significantly reduced the production of TNF-α in response to BLP in contrast to macrophages from wild type mice. We conclude, BLP acting through TLR2, is a potent inducer of inflammation with a response profile both common and distinct from LPS. Hence, BLP mediated pathway may also be considered as an effective target against sepsis. Nature Publishing Group 2016-10-04 /pmc/articles/PMC5048175/ /pubmed/27698491 http://dx.doi.org/10.1038/srep34666 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lakshmikanth, Chikkamenahalli Lakshminarayana
Jacob, Shancy Petsel
Kudva, Avinash Kundadka
Latchoumycandane, Calivarathan
Yashaswini, Puttaraju Srikanta Murthy
Sumanth, Mosale Seetharam
Goncalves-de-Albuquerque, Cassiano F.
Silva, Adriana R.
Singh, Sridevi Annapurna
Castro-Faria-Neto, Hugo C.
Prabhu, Sandeep Kumble
McIntyre, Thomas M.
Marathe, Gopal Kedihithlu
Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice
title Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice
title_full Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice
title_fullStr Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice
title_full_unstemmed Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice
title_short Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice
title_sort escherichia coli braun lipoprotein (blp) exhibits endotoxemia – like pathology in swiss albino mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048175/
https://www.ncbi.nlm.nih.gov/pubmed/27698491
http://dx.doi.org/10.1038/srep34666
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