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Alpha‐synuclein RT‐QuIC in the CSF of patients with alpha‐synucleinopathies
We have developed a novel real‐time quaking‐induced conversion RT‐QuIC‐based assay to detect alpha‐synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha‐synuclein aggregation in Dementia with Lewy bodi...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048391/ https://www.ncbi.nlm.nih.gov/pubmed/27752516 http://dx.doi.org/10.1002/acn3.338 |
| _version_ | 1782457580179685376 |
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| author | Fairfoul, Graham McGuire, Lynne I. Pal, Suvankar Ironside, James W. Neumann, Juliane Christie, Sharon Joachim, Catherine Esiri, Margaret Evetts, Samuel G. Rolinski, Michal Baig, Fahd Ruffmann, Claudio Wade‐Martins, Richard Hu, Michele T. M. Parkkinen, Laura Green, Alison J. E. |
| author_facet | Fairfoul, Graham McGuire, Lynne I. Pal, Suvankar Ironside, James W. Neumann, Juliane Christie, Sharon Joachim, Catherine Esiri, Margaret Evetts, Samuel G. Rolinski, Michal Baig, Fahd Ruffmann, Claudio Wade‐Martins, Richard Hu, Michele T. M. Parkkinen, Laura Green, Alison J. E. |
| author_sort | Fairfoul, Graham |
| collection | PubMed |
| description | We have developed a novel real‐time quaking‐induced conversion RT‐QuIC‐based assay to detect alpha‐synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha‐synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT‐QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha‐synucleinopathies. |
| format | Online Article Text |
| id | pubmed-5048391 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2016 |
| publisher | John Wiley and Sons Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-50483912016-10-17 Alpha‐synuclein RT‐QuIC in the CSF of patients with alpha‐synucleinopathies Fairfoul, Graham McGuire, Lynne I. Pal, Suvankar Ironside, James W. Neumann, Juliane Christie, Sharon Joachim, Catherine Esiri, Margaret Evetts, Samuel G. Rolinski, Michal Baig, Fahd Ruffmann, Claudio Wade‐Martins, Richard Hu, Michele T. M. Parkkinen, Laura Green, Alison J. E. Ann Clin Transl Neurol Brief Communications We have developed a novel real‐time quaking‐induced conversion RT‐QuIC‐based assay to detect alpha‐synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha‐synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT‐QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha‐synucleinopathies. John Wiley and Sons Inc. 2016-08-28 /pmc/articles/PMC5048391/ /pubmed/27752516 http://dx.doi.org/10.1002/acn3.338 Text en © 2016 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
| spellingShingle | Brief Communications Fairfoul, Graham McGuire, Lynne I. Pal, Suvankar Ironside, James W. Neumann, Juliane Christie, Sharon Joachim, Catherine Esiri, Margaret Evetts, Samuel G. Rolinski, Michal Baig, Fahd Ruffmann, Claudio Wade‐Martins, Richard Hu, Michele T. M. Parkkinen, Laura Green, Alison J. E. Alpha‐synuclein RT‐QuIC in the CSF of patients with alpha‐synucleinopathies |
| title | Alpha‐synuclein RT‐QuIC in the CSF of patients with alpha‐synucleinopathies |
| title_full | Alpha‐synuclein RT‐QuIC in the CSF of patients with alpha‐synucleinopathies |
| title_fullStr | Alpha‐synuclein RT‐QuIC in the CSF of patients with alpha‐synucleinopathies |
| title_full_unstemmed | Alpha‐synuclein RT‐QuIC in the CSF of patients with alpha‐synucleinopathies |
| title_short | Alpha‐synuclein RT‐QuIC in the CSF of patients with alpha‐synucleinopathies |
| title_sort | alpha‐synuclein rt‐quic in the csf of patients with alpha‐synucleinopathies |
| topic | Brief Communications |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048391/ https://www.ncbi.nlm.nih.gov/pubmed/27752516 http://dx.doi.org/10.1002/acn3.338 |
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