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Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus

To understand the impact of a hypovirus infection on the secretome of the chestnut blight fungus, Cryphonectria parasitica, a phytopathogenic filamentous fungus, two-dimensional electrophoresis (2-DE) and isobaric tag for relative and absolute quantitation (iTRAQ) technology were employed to identif...

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Autores principales: Wang, Jinzi, Shi, Liming, He, Xipu, Lu, Lidan, Li, Xiaoping, Chen, Baoshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048421/
https://www.ncbi.nlm.nih.gov/pubmed/27698384
http://dx.doi.org/10.1038/srep34308
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author Wang, Jinzi
Shi, Liming
He, Xipu
Lu, Lidan
Li, Xiaoping
Chen, Baoshan
author_facet Wang, Jinzi
Shi, Liming
He, Xipu
Lu, Lidan
Li, Xiaoping
Chen, Baoshan
author_sort Wang, Jinzi
collection PubMed
description To understand the impact of a hypovirus infection on the secretome of the chestnut blight fungus, Cryphonectria parasitica, a phytopathogenic filamentous fungus, two-dimensional electrophoresis (2-DE) and isobaric tag for relative and absolute quantitation (iTRAQ) technology were employed to identify and quantify the secreted proteins. A total of 403 unique proteins were identified from the secretome of the wild type virus-free strain EP155. Of these proteins, 329 were predicted to be involved in known secretory pathways and they are primarily composed of metabolic enzymes, biological regulators, responders to stimulus and components involved in plant-pathogen interactions. When infected with the hypovirus CHV1-EP713, 99 proteins were found to be differentially expressed as compared to the wild type strain EP155. These proteins were mainly related to plant cell wall degradation, response to host defense, fungal virulence and intracellular structure. The effects of CHV1 on secreted proteins may reveal a relationship between physiological pathways and hypovirulence.
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spelling pubmed-50484212016-10-11 Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus Wang, Jinzi Shi, Liming He, Xipu Lu, Lidan Li, Xiaoping Chen, Baoshan Sci Rep Article To understand the impact of a hypovirus infection on the secretome of the chestnut blight fungus, Cryphonectria parasitica, a phytopathogenic filamentous fungus, two-dimensional electrophoresis (2-DE) and isobaric tag for relative and absolute quantitation (iTRAQ) technology were employed to identify and quantify the secreted proteins. A total of 403 unique proteins were identified from the secretome of the wild type virus-free strain EP155. Of these proteins, 329 were predicted to be involved in known secretory pathways and they are primarily composed of metabolic enzymes, biological regulators, responders to stimulus and components involved in plant-pathogen interactions. When infected with the hypovirus CHV1-EP713, 99 proteins were found to be differentially expressed as compared to the wild type strain EP155. These proteins were mainly related to plant cell wall degradation, response to host defense, fungal virulence and intracellular structure. The effects of CHV1 on secreted proteins may reveal a relationship between physiological pathways and hypovirulence. Nature Publishing Group 2016-10-04 /pmc/articles/PMC5048421/ /pubmed/27698384 http://dx.doi.org/10.1038/srep34308 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Jinzi
Shi, Liming
He, Xipu
Lu, Lidan
Li, Xiaoping
Chen, Baoshan
Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus
title Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus
title_full Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus
title_fullStr Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus
title_full_unstemmed Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus
title_short Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus
title_sort comparative secretome analysis reveals perturbation of host secretion pathways by a hypovirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048421/
https://www.ncbi.nlm.nih.gov/pubmed/27698384
http://dx.doi.org/10.1038/srep34308
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