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Repeated superovulation may affect mitochondrial functions of cumulus cells in mice
Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated super...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048428/ https://www.ncbi.nlm.nih.gov/pubmed/27698360 http://dx.doi.org/10.1038/srep31368 |
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author | Xie, Juan-Ke Wang, Qian Zhang, Ting-Ting Yin, Shen Zhang, Cui-Lian Ge, Zhao-Jia |
author_facet | Xie, Juan-Ke Wang, Qian Zhang, Ting-Ting Yin, Shen Zhang, Cui-Lian Ge, Zhao-Jia |
author_sort | Xie, Juan-Ke |
collection | PubMed |
description | Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated superovulation adversely affects the mitochondrial functions of cumulus cells remains unclear. In this study, mice were divided into three groups: superovulation once (R1); superovulation three times (R3), and superovulation five times (R5). We evaluated the effects of repeated superovulation on mitochondrial DNA copies (mtDNA) and observed decreased mtDNA copies per cell with increasing number of superovulation cycles. Further, we investigated the DNA methylation status in exon 2 and the mRNA expression level of nuclear-encoded DNA polymerase gamma A (PolgA). The results showed that the DNA methylation levels of PolgA in R1 and R5 were slightly lower than in R3. Additionally, the altered DNA methylation in PolgA coincided with the changes in PolgA expression in cumulus cells. We also found that the mRNA expression of COX1, CYTB, ND2, and ND4 was altered by repeated superovulation in cumulus cells. Thus, repeated superovulation had adverse effects on mitochondrial function. |
format | Online Article Text |
id | pubmed-5048428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50484282016-10-11 Repeated superovulation may affect mitochondrial functions of cumulus cells in mice Xie, Juan-Ke Wang, Qian Zhang, Ting-Ting Yin, Shen Zhang, Cui-Lian Ge, Zhao-Jia Sci Rep Article Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated superovulation adversely affects the mitochondrial functions of cumulus cells remains unclear. In this study, mice were divided into three groups: superovulation once (R1); superovulation three times (R3), and superovulation five times (R5). We evaluated the effects of repeated superovulation on mitochondrial DNA copies (mtDNA) and observed decreased mtDNA copies per cell with increasing number of superovulation cycles. Further, we investigated the DNA methylation status in exon 2 and the mRNA expression level of nuclear-encoded DNA polymerase gamma A (PolgA). The results showed that the DNA methylation levels of PolgA in R1 and R5 were slightly lower than in R3. Additionally, the altered DNA methylation in PolgA coincided with the changes in PolgA expression in cumulus cells. We also found that the mRNA expression of COX1, CYTB, ND2, and ND4 was altered by repeated superovulation in cumulus cells. Thus, repeated superovulation had adverse effects on mitochondrial function. Nature Publishing Group 2016-10-04 /pmc/articles/PMC5048428/ /pubmed/27698360 http://dx.doi.org/10.1038/srep31368 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Xie, Juan-Ke Wang, Qian Zhang, Ting-Ting Yin, Shen Zhang, Cui-Lian Ge, Zhao-Jia Repeated superovulation may affect mitochondrial functions of cumulus cells in mice |
title | Repeated superovulation may affect mitochondrial functions of cumulus cells in mice |
title_full | Repeated superovulation may affect mitochondrial functions of cumulus cells in mice |
title_fullStr | Repeated superovulation may affect mitochondrial functions of cumulus cells in mice |
title_full_unstemmed | Repeated superovulation may affect mitochondrial functions of cumulus cells in mice |
title_short | Repeated superovulation may affect mitochondrial functions of cumulus cells in mice |
title_sort | repeated superovulation may affect mitochondrial functions of cumulus cells in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048428/ https://www.ncbi.nlm.nih.gov/pubmed/27698360 http://dx.doi.org/10.1038/srep31368 |
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