Genetically encoded optical activation of DNA recombination in human cells

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial cont...

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Detalles Bibliográficos
Autores principales: Luo, J., Arbely, E., Zhang, J., Chou, C., Uprety, R., Chin, J. W., Deiters, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048445/
https://www.ncbi.nlm.nih.gov/pubmed/27277957
http://dx.doi.org/10.1039/c6cc03934k
Descripción
Sumario:We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

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