Genetically encoded optical activation of DNA recombination in human cells

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial cont...

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Detalles Bibliográficos
Autores principales: Luo, J., Arbely, E., Zhang, J., Chou, C., Uprety, R., Chin, J. W., Deiters, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048445/
https://www.ncbi.nlm.nih.gov/pubmed/27277957
http://dx.doi.org/10.1039/c6cc03934k
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author Luo, J.
Arbely, E.
Zhang, J.
Chou, C.
Uprety, R.
Chin, J. W.
Deiters, A.
author_facet Luo, J.
Arbely, E.
Zhang, J.
Chou, C.
Uprety, R.
Chin, J. W.
Deiters, A.
author_sort Luo, J.
collection PubMed
description We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.
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spelling pubmed-50484452016-10-12 Genetically encoded optical activation of DNA recombination in human cells Luo, J. Arbely, E. Zhang, J. Chou, C. Uprety, R. Chin, J. W. Deiters, A. Chem Commun (Camb) Chemistry We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination. Royal Society of Chemistry 2016-07-14 2016-06-09 /pmc/articles/PMC5048445/ /pubmed/27277957 http://dx.doi.org/10.1039/c6cc03934k Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Luo, J.
Arbely, E.
Zhang, J.
Chou, C.
Uprety, R.
Chin, J. W.
Deiters, A.
Genetically encoded optical activation of DNA recombination in human cells
title Genetically encoded optical activation of DNA recombination in human cells
title_full Genetically encoded optical activation of DNA recombination in human cells
title_fullStr Genetically encoded optical activation of DNA recombination in human cells
title_full_unstemmed Genetically encoded optical activation of DNA recombination in human cells
title_short Genetically encoded optical activation of DNA recombination in human cells
title_sort genetically encoded optical activation of dna recombination in human cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048445/
https://www.ncbi.nlm.nih.gov/pubmed/27277957
http://dx.doi.org/10.1039/c6cc03934k
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