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Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion

BACKGROUND: The aim of this study was to investigate the possible associations of miRNA-27a and Leptin polymorphisms with the risk of recurrent spontaneous abortion (RSA). MATERIAL/METHODS: Between May 2013 and April 2015 at Shenzhen Longhua New District Central Hospital, we randomly recruited 138 R...

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Autores principales: Wang, Chun-Yan, Wang, Shu-Guang, Wang, Jia-Li, Zhou, Li-Ying, Liu, Hong-Jun, Wang, Yi-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5049305/
https://www.ncbi.nlm.nih.gov/pubmed/27694792
http://dx.doi.org/10.12659/MSM.897147
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author Wang, Chun-Yan
Wang, Shu-Guang
Wang, Jia-Li
Zhou, Li-Ying
Liu, Hong-Jun
Wang, Yi-Feng
author_facet Wang, Chun-Yan
Wang, Shu-Guang
Wang, Jia-Li
Zhou, Li-Ying
Liu, Hong-Jun
Wang, Yi-Feng
author_sort Wang, Chun-Yan
collection PubMed
description BACKGROUND: The aim of this study was to investigate the possible associations of miRNA-27a and Leptin polymorphisms with the risk of recurrent spontaneous abortion (RSA). MATERIAL/METHODS: Between May 2013 and April 2015 at Shenzhen Longhua New District Central Hospital, we randomly recruited 138 RSA patients as the case group and another 142 normal pregnancy women as the control group. We used denaturing high-performance liquid chromatography (DHPLC) to determine the genotypes and allele frequencies of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A. RESULTS: The GG genotype and G allele frequencies of miRNA-27a rs895819 A/G were higher in the case group than in the control group, and the AA genotype and A allele frequencies of Leptin rs7799039 G/A were also higher in the case group than in the control group (all P<0.05). MiRNA-27a rs895819 A/G and Leptin rs7799039 G/A polymorphisms increased the risk of RSA (Exp (B)=2.732, 95% CI=1.625~4.596, P=0.000; Exp (B)=4.081, 95% CI=1.817~9.164, P=0.001). GG-AA or AG-AA carriers had a higher risk of RSA. The miRNA-27a expression of AA carriers of miRNA-27a rs895819 was lower than that of AG+GG carriers both in the case and control groups (all P=0.024). The plasma leptin concentration of GG carriers was lower than that of GA+AA carriers in the case group (P=0.026). CONCLUSIONS: The polymorphisms of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A may contribute to an increased risk of RSA.
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spelling pubmed-50493052016-10-18 Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion Wang, Chun-Yan Wang, Shu-Guang Wang, Jia-Li Zhou, Li-Ying Liu, Hong-Jun Wang, Yi-Feng Med Sci Monit Clinical Research BACKGROUND: The aim of this study was to investigate the possible associations of miRNA-27a and Leptin polymorphisms with the risk of recurrent spontaneous abortion (RSA). MATERIAL/METHODS: Between May 2013 and April 2015 at Shenzhen Longhua New District Central Hospital, we randomly recruited 138 RSA patients as the case group and another 142 normal pregnancy women as the control group. We used denaturing high-performance liquid chromatography (DHPLC) to determine the genotypes and allele frequencies of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A. RESULTS: The GG genotype and G allele frequencies of miRNA-27a rs895819 A/G were higher in the case group than in the control group, and the AA genotype and A allele frequencies of Leptin rs7799039 G/A were also higher in the case group than in the control group (all P<0.05). MiRNA-27a rs895819 A/G and Leptin rs7799039 G/A polymorphisms increased the risk of RSA (Exp (B)=2.732, 95% CI=1.625~4.596, P=0.000; Exp (B)=4.081, 95% CI=1.817~9.164, P=0.001). GG-AA or AG-AA carriers had a higher risk of RSA. The miRNA-27a expression of AA carriers of miRNA-27a rs895819 was lower than that of AG+GG carriers both in the case and control groups (all P=0.024). The plasma leptin concentration of GG carriers was lower than that of GA+AA carriers in the case group (P=0.026). CONCLUSIONS: The polymorphisms of miRNA-27a rs895819 A/G and Leptin rs7799039 G/A may contribute to an increased risk of RSA. International Scientific Literature, Inc. 2016-10-02 /pmc/articles/PMC5049305/ /pubmed/27694792 http://dx.doi.org/10.12659/MSM.897147 Text en © Med Sci Monit, 2016 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
spellingShingle Clinical Research
Wang, Chun-Yan
Wang, Shu-Guang
Wang, Jia-Li
Zhou, Li-Ying
Liu, Hong-Jun
Wang, Yi-Feng
Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion
title Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion
title_full Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion
title_fullStr Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion
title_full_unstemmed Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion
title_short Effect of miRNA-27a and Leptin Polymorphisms on Risk of Recurrent Spontaneous Abortion
title_sort effect of mirna-27a and leptin polymorphisms on risk of recurrent spontaneous abortion
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5049305/
https://www.ncbi.nlm.nih.gov/pubmed/27694792
http://dx.doi.org/10.12659/MSM.897147
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