Cargando…
Fast and Efficient Transfection of Mouse Embryonic Stem Cells Using Non-Viral Reagents
Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. We have found that transfecti...
Autores principales: | Tamm, Christoffer, Kadekar, Sandeep, Pijuan-Galitó, Sara, Annerén, Cecilia |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050252/ https://www.ncbi.nlm.nih.gov/pubmed/27358240 http://dx.doi.org/10.1007/s12015-016-9673-5 |
Ejemplares similares
-
A Comparative Study of Protocols for Mouse Embryonic Stem Cell Culturing
por: Tamm, Christoffer, et al.
Publicado: (2013) -
Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
por: Pijuan-Galitó, Sara, et al.
Publicado: (2016) -
Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency
por: Sork, Helena, et al.
Publicado: (2016) -
The challenging nature of primary T lymphocytes for transfection: Effect of protamine sulfate on the transfection efficiency of chemical transfection reagents
por: Rahimmanesh, Ilnaz, et al.
Publicado: (2020) -
Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent
por: Bodbin, Sara E., et al.
Publicado: (2020)