Cargando…
Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia
Males of all species of the parasitic wasp genus Nasonia use (4R,5S)-5-hydroxy-4-decanolide (RS) as component of their sex pheromone while only N. vitripennis (Nv), employs additionally (4R,5R)-5-hydroxy-4-decanolide (RR). Three genes coding for the NAD(+)-dependent short-chain dehydrogenases/reduct...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050451/ https://www.ncbi.nlm.nih.gov/pubmed/27703264 http://dx.doi.org/10.1038/srep34697 |
_version_ | 1782457885058400256 |
---|---|
author | Ruther, Joachim Hagström, Åsa K. Brandstetter, Birgit Hofferberth, John Bruckmann, Astrid Semmelmann, Florian Fink, Michaela Lowack, Helena Laberer, Sabine Niehuis, Oliver Deutzmann, Rainer Löfstedt, Christer Sterner, Reinhard |
author_facet | Ruther, Joachim Hagström, Åsa K. Brandstetter, Birgit Hofferberth, John Bruckmann, Astrid Semmelmann, Florian Fink, Michaela Lowack, Helena Laberer, Sabine Niehuis, Oliver Deutzmann, Rainer Löfstedt, Christer Sterner, Reinhard |
author_sort | Ruther, Joachim |
collection | PubMed |
description | Males of all species of the parasitic wasp genus Nasonia use (4R,5S)-5-hydroxy-4-decanolide (RS) as component of their sex pheromone while only N. vitripennis (Nv), employs additionally (4R,5R)-5-hydroxy-4-decanolide (RR). Three genes coding for the NAD(+)-dependent short-chain dehydrogenases/reductases (SDRs) NV10127, NV10128, and NV10129 are linked to the ability of Nv to produce RR. Here we show by assaying recombinant enzymes that SDRs from both Nv and N. giraulti (Ng), the latter a species with only RS in the pheromone, epimerise RS into RR and vice versa with (4R)-5-oxo-4-decanolide as an intermediate. Nv-derived SDR orthologues generally had higher epimerisation rates, which were also influenced by NAD(+) availability. Semiquantitative protein analyses of the pheromone glands by tandem mass spectrometry revealed that NV10127 as well as NV10128 and/or NV10129 were more abundant in Nv compared to Ng. We conclude that the interplay of differential expression patterns and SDR epimerisation rates on the ancestral pheromone component RS accounts for the evolution of a novel pheromone phenotype in Nv. |
format | Online Article Text |
id | pubmed-5050451 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50504512016-10-11 Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia Ruther, Joachim Hagström, Åsa K. Brandstetter, Birgit Hofferberth, John Bruckmann, Astrid Semmelmann, Florian Fink, Michaela Lowack, Helena Laberer, Sabine Niehuis, Oliver Deutzmann, Rainer Löfstedt, Christer Sterner, Reinhard Sci Rep Article Males of all species of the parasitic wasp genus Nasonia use (4R,5S)-5-hydroxy-4-decanolide (RS) as component of their sex pheromone while only N. vitripennis (Nv), employs additionally (4R,5R)-5-hydroxy-4-decanolide (RR). Three genes coding for the NAD(+)-dependent short-chain dehydrogenases/reductases (SDRs) NV10127, NV10128, and NV10129 are linked to the ability of Nv to produce RR. Here we show by assaying recombinant enzymes that SDRs from both Nv and N. giraulti (Ng), the latter a species with only RS in the pheromone, epimerise RS into RR and vice versa with (4R)-5-oxo-4-decanolide as an intermediate. Nv-derived SDR orthologues generally had higher epimerisation rates, which were also influenced by NAD(+) availability. Semiquantitative protein analyses of the pheromone glands by tandem mass spectrometry revealed that NV10127 as well as NV10128 and/or NV10129 were more abundant in Nv compared to Ng. We conclude that the interplay of differential expression patterns and SDR epimerisation rates on the ancestral pheromone component RS accounts for the evolution of a novel pheromone phenotype in Nv. Nature Publishing Group 2016-10-05 /pmc/articles/PMC5050451/ /pubmed/27703264 http://dx.doi.org/10.1038/srep34697 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ruther, Joachim Hagström, Åsa K. Brandstetter, Birgit Hofferberth, John Bruckmann, Astrid Semmelmann, Florian Fink, Michaela Lowack, Helena Laberer, Sabine Niehuis, Oliver Deutzmann, Rainer Löfstedt, Christer Sterner, Reinhard Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia |
title | Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia |
title_full | Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia |
title_fullStr | Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia |
title_full_unstemmed | Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia |
title_short | Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia |
title_sort | epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in nasonia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050451/ https://www.ncbi.nlm.nih.gov/pubmed/27703264 http://dx.doi.org/10.1038/srep34697 |
work_keys_str_mv | AT rutherjoachim epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT hagstromasak epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT brandstetterbirgit epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT hofferberthjohn epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT bruckmannastrid epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT semmelmannflorian epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT finkmichaela epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT lowackhelena epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT laberersabine epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT niehuisoliver epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT deutzmannrainer epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT lofstedtchrister epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia AT sternerreinhard epimerisationofchiralhydroxylactonesbyshortchaindehydrogenasesreductasesaccountsforsexpheromoneevolutioninnasonia |