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Preparation of cross-linked hen-egg white lysozyme crystals free of cracks
Cross-linked protein crystals (CLPCs) are very useful materials in applications such as biosensors, catalysis, and X-ray crystallography. Hence, preparation of CLPCs is an important research direction. During the preparation of CLPCs, an often encountered problem is that cracks may appear in the cry...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050519/ https://www.ncbi.nlm.nih.gov/pubmed/27703210 http://dx.doi.org/10.1038/srep34770 |
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author | Yan, Er-Kai Lu, Qin-Qin Zhang, Chen-Yan Liu, Ya-Li He, Jin Chen, Da Wang, Bo Zhou, Ren-Bin Wu, Ping Yin, Da-Chuan |
author_facet | Yan, Er-Kai Lu, Qin-Qin Zhang, Chen-Yan Liu, Ya-Li He, Jin Chen, Da Wang, Bo Zhou, Ren-Bin Wu, Ping Yin, Da-Chuan |
author_sort | Yan, Er-Kai |
collection | PubMed |
description | Cross-linked protein crystals (CLPCs) are very useful materials in applications such as biosensors, catalysis, and X-ray crystallography. Hence, preparation of CLPCs is an important research direction. During the preparation of CLPCs, an often encountered problem is that cracks may appear in the crystals, which may finally lead to shattering of the crystals into small pieces and cause problem in practical applications. To avoid cross-link induced cracking, it is necessary to study the cracking phenomenon in the preparation process. In this paper, we present an investigation on how to avoid cracking during preparation of CLPCs. An orthogonal experiment was designed to study the phenomenon of cross-link induced cracking of hen-egg white lysozyme (HEWL) crystals against five parameters (temperature, solution pH, crystal growth time, glutaraldehyde concentration, and cross-linking time). The experimental results showed that, the solution pH and crystal growth time can significantly affect cross-link induced cracking. The possible mechanism was studied, and optimized conditions for obtaining crack-free CLPCs were obtained and experimentally verified. |
format | Online Article Text |
id | pubmed-5050519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50505192016-10-11 Preparation of cross-linked hen-egg white lysozyme crystals free of cracks Yan, Er-Kai Lu, Qin-Qin Zhang, Chen-Yan Liu, Ya-Li He, Jin Chen, Da Wang, Bo Zhou, Ren-Bin Wu, Ping Yin, Da-Chuan Sci Rep Article Cross-linked protein crystals (CLPCs) are very useful materials in applications such as biosensors, catalysis, and X-ray crystallography. Hence, preparation of CLPCs is an important research direction. During the preparation of CLPCs, an often encountered problem is that cracks may appear in the crystals, which may finally lead to shattering of the crystals into small pieces and cause problem in practical applications. To avoid cross-link induced cracking, it is necessary to study the cracking phenomenon in the preparation process. In this paper, we present an investigation on how to avoid cracking during preparation of CLPCs. An orthogonal experiment was designed to study the phenomenon of cross-link induced cracking of hen-egg white lysozyme (HEWL) crystals against five parameters (temperature, solution pH, crystal growth time, glutaraldehyde concentration, and cross-linking time). The experimental results showed that, the solution pH and crystal growth time can significantly affect cross-link induced cracking. The possible mechanism was studied, and optimized conditions for obtaining crack-free CLPCs were obtained and experimentally verified. Nature Publishing Group 2016-10-05 /pmc/articles/PMC5050519/ /pubmed/27703210 http://dx.doi.org/10.1038/srep34770 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Yan, Er-Kai Lu, Qin-Qin Zhang, Chen-Yan Liu, Ya-Li He, Jin Chen, Da Wang, Bo Zhou, Ren-Bin Wu, Ping Yin, Da-Chuan Preparation of cross-linked hen-egg white lysozyme crystals free of cracks |
title | Preparation of cross-linked hen-egg white lysozyme crystals free of cracks |
title_full | Preparation of cross-linked hen-egg white lysozyme crystals free of cracks |
title_fullStr | Preparation of cross-linked hen-egg white lysozyme crystals free of cracks |
title_full_unstemmed | Preparation of cross-linked hen-egg white lysozyme crystals free of cracks |
title_short | Preparation of cross-linked hen-egg white lysozyme crystals free of cracks |
title_sort | preparation of cross-linked hen-egg white lysozyme crystals free of cracks |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050519/ https://www.ncbi.nlm.nih.gov/pubmed/27703210 http://dx.doi.org/10.1038/srep34770 |
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