Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly

Malonyl carba(dethia) N-decanoyl cysteamine methyl esters and novel acetoxymethyl esters were utilised as second-generation probes for polyketide intermediate capture. The use of these tools in vivo led to the characterisation of an almost complete set of biosynthetic intermediates from a modular as...

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Detalles Bibliográficos
Autores principales: Wilkening, Ina, Gazzola, Silvia, Riva, Elena, Parascandolo, James S., Song, Lijiang, Tosin, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050551/
https://www.ncbi.nlm.nih.gov/pubmed/27481638
http://dx.doi.org/10.1039/c6cc04681a
Descripción
Sumario:Malonyl carba(dethia) N-decanoyl cysteamine methyl esters and novel acetoxymethyl esters were utilised as second-generation probes for polyketide intermediate capture. The use of these tools in vivo led to the characterisation of an almost complete set of biosynthetic intermediates from a modular assembly line, providing a first kinetic overview of intermediate processing leading to complex natural product formation.

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