Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly

Malonyl carba(dethia) N-decanoyl cysteamine methyl esters and novel acetoxymethyl esters were utilised as second-generation probes for polyketide intermediate capture. The use of these tools in vivo led to the characterisation of an almost complete set of biosynthetic intermediates from a modular as...

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Autores principales: Wilkening, Ina, Gazzola, Silvia, Riva, Elena, Parascandolo, James S., Song, Lijiang, Tosin, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050551/
https://www.ncbi.nlm.nih.gov/pubmed/27481638
http://dx.doi.org/10.1039/c6cc04681a
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author Wilkening, Ina
Gazzola, Silvia
Riva, Elena
Parascandolo, James S.
Song, Lijiang
Tosin, Manuela
author_facet Wilkening, Ina
Gazzola, Silvia
Riva, Elena
Parascandolo, James S.
Song, Lijiang
Tosin, Manuela
author_sort Wilkening, Ina
collection PubMed
description Malonyl carba(dethia) N-decanoyl cysteamine methyl esters and novel acetoxymethyl esters were utilised as second-generation probes for polyketide intermediate capture. The use of these tools in vivo led to the characterisation of an almost complete set of biosynthetic intermediates from a modular assembly line, providing a first kinetic overview of intermediate processing leading to complex natural product formation.
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spelling pubmed-50505512016-10-12 Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly Wilkening, Ina Gazzola, Silvia Riva, Elena Parascandolo, James S. Song, Lijiang Tosin, Manuela Chem Commun (Camb) Chemistry Malonyl carba(dethia) N-decanoyl cysteamine methyl esters and novel acetoxymethyl esters were utilised as second-generation probes for polyketide intermediate capture. The use of these tools in vivo led to the characterisation of an almost complete set of biosynthetic intermediates from a modular assembly line, providing a first kinetic overview of intermediate processing leading to complex natural product formation. Royal Society of Chemistry 2016-09-04 2016-08-02 /pmc/articles/PMC5050551/ /pubmed/27481638 http://dx.doi.org/10.1039/c6cc04681a Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Wilkening, Ina
Gazzola, Silvia
Riva, Elena
Parascandolo, James S.
Song, Lijiang
Tosin, Manuela
Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly
title Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly
title_full Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly
title_fullStr Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly
title_full_unstemmed Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly
title_short Second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly
title_sort second-generation probes for biosynthetic intermediate capture: towards a comprehensive profiling of polyketide assembly
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050551/
https://www.ncbi.nlm.nih.gov/pubmed/27481638
http://dx.doi.org/10.1039/c6cc04681a
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